1887

Abstract

QseE is a sensor kinase that responds to epinephrine, sulfate and phosphate. QseE constitutes a two-component signalling system together with the QseF -dependent response regulator. Encoded within the same operon as is the gene, which encodes a membrane protein involved in the translocation of a type III secretion effector protein of enterohaemorrhagic (EHEC) into epithelial cells. The genes also form an operon with the gene, which encodes the nitrogen sensor PII protein. Here we report a transcriptome analysis comparing , and single mutants with the wild-type strain. This study revealed that the proteins encoded by these genes play a modest but significant role in iron uptake. Although QseEFG regulate genes involved in nitrogen utilization, these proteins do not play a notable role in nitrogen metabolism. In addition, QseEFG regulate transcription of the and two-component systems, linking several signal transduction pathways. The similarity of the microarray profiles of these mutants also indicates that these proteins work together. These data indicate that QseEFG are involved in the regulation of virulence and metabolism in EHEC.

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2010-04-01
2020-01-17
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