1887

Abstract

The BpeAB-OprB resistance-nodulation-division (RND) family pump effluxes aminoglycoside and macrolide antibiotics as well as acylhomoserine lactones (AHLs) involved in quorum sensing. Expression of was cell density-dependent and was inducible in the presence of these compounds. Intracellular levels of spermidine and -acetylspermidine increased with cell density in wild-type KHW, but were always lower in the pump mutant at all growth phases. The significance of changes in intracellular spermidine on efflux pump expression was demonstrated by the disruption of the binding of the BpeR repressor protein to the regulatory region in the presence of increasing spermidine concentrations. This was supported by dose-dependent activation of transcription in the presence of exogenous spermidine and -acetylspermidine, thus implicating the involvement of the BpeAB-OprB pump in spermidine homeostasis in . Consequently, inhibition of intracellular spermidine synthesis reduced the efflux of AHLs by BpeAB-OprB. Other potential therapeutic applications of spermidine synthase inhibitors include the reduction of swimming motility and biofilm formation by .

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2010-04-01
2019-10-15
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