1887

Abstract

We have isolated serine protease inhibitors from the basidiomycete , CnSPIs, using trypsin affinity chromatography. Full-length gene and cDNA sequences were determined for one of them, named cnispin, and the recombinant protein was expressed in at high yield. The primary structure and biochemical properties of cnispin are very similar to those of the serine protease inhibitor, until now the only member of the I66 family of protease inhibitors in the MEROPS classification. Cnispin is highly specific towards trypsin, with in the nanomolar range. It also exhibited weaker inhibition of chymotrypsin and very weak inhibition of subtilisin and kallikrein; other proteases were not inhibited. Inhibitory activity against endogenous proteases from . revealed a possible regulatory role for CnSPIs in the endogenous proteolytic system. Another possible biological function in defence against predatory insects was indicated by the deleterious effect of CnSPIs on the development of larvae of . These findings, together with the biochemical and genetic characterization of cnispin, suggest a dual physiological role for this serine protease inhibitor of the I66 MEROPS family.

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2009-12-01
2019-10-23
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vol. , part 12, pp. 3971 - 3981

[ PDF, 418 kb], containing: List of degenerate and specific primers used in PCRs Size-exclusion chromatography of crude protein extract from on a Sepharose S-200 column Far-UV CD spectrum of recombinant cnipsin (rCnp) Thermal stability of recombinant cnipsin (rCnp)



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