RT Journal Article SR Electronic(1) A1 Evans, T. J. A1 Crow, M. A. A1 Williamson, N. R. A1 Orme, W. A1 Thomson, N. R. A1 Komitopoulou, E. A1 Salmond, G. P. C.YR 2010 T1 Characterization of a broad-host-range flagellum-dependent phage that mediates high-efficiency generalized transduction in, and between, Serratia and Pantoea JF Microbiology, VO 156 IS 1 SP 240 OP 247 DO https://doi.org/10.1099/mic.0.032797-0 PB Microbiology Society, SN 1465-2080, AB A phage (ΦOT8) isolated on Serratia sp. ATCC 39006 was shown to be flagellum-dependent, and to mediate generalized transduction with high efficiency (up to 10−4 transductants per p.f.u.). ΦOT8 was shown to have a broad host range because it also infected a strain of Pantoea agglomerans isolated from the rhizosphere. Transduction of plasmid-borne antibiotic resistance between the two bacterial genera was demonstrated, consistent with purported ecological roles of phages in dissemination of genes between bacterial genera. Serratia sp. ATCC 39006 and P. agglomerans produce a number of interesting secondary metabolites that have potential applications in cancer therapy and biocontrol of fungal infections. ΦOT8 has utility as a powerful functional genomics tool in these bacteria., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.032797-0