1887

Abstract

Maggot debridement therapy (MDT) is widely used for debridement of chronic infected wounds; however, for wounds harbouring specific bacteria limited effect or failure of the treatment has been described. Here we studied the survival of maggots encountering PAO1 in a simple assay with emphasis on the quorum-sensing (QS)-regulated virulence. The maggots were challenged with GFP-tagged wild-type (WT) PAO1 and a GFP-tagged Δ (ΔRR) QS-deficient mutant in different concentrations. Maggots were killed in the presence of WT PAO1 whereas the challenge with the QS mutant showed a survival reduction of ∼25 % compared to negative controls. Furthermore, bacterial intake by the maggots was lower in the presence of WT PAO1 compared to the PAO1 ΔRR mutant. Maggot excretions/secretions (ES) were assayed for the presence of QS inhibitors; only high doses of ES showed inhibition of QS in . Thus was shown to be toxic to maggots. This, coupled to the preferential feeding by the maggots and reduced ingestion of , could explain MDT failure in wounds colonized by Wounds heavily colonized with should be a counterindication for MDT unless used in combination with a pre-treatment with other topical therapeutics targeting .

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2010-02-01
2019-12-07
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