Elevated levels of inhibit biofilm formation in : a role for the Rcs phosphorelay Free

Abstract

The Rcs phosphorelay is composed of RcsC, RcsD and the response regulator RcsB, and this signalling pathway has been implicated in virulence and biofilm formation in many enteric bacteria. It was previously shown that a mutation in resulted in defective biofilm formation in [ Ferrières, L. & Clarke, D. J. (2003) , 1665–1682 ]. To identify the molecular mechanisms underlying the observed biofilm defect we carried out a screen looking for suppressor mutants that restored biofilm formation in the mutant background. One of the mutants was identified to be in , a gene encoding a small RNA molecule that is involved in the post-transcriptional control of the alternative sigma factor, . The expression of is regulated by the Rcs phosphorelay, and there are elevated levels present in the mutant due to the overexpression of in this background. Using different approaches, we have established that the increase in levels is responsible for the biofilm defect. Therefore, the Rcs phosphorelay is involved in maintaining appropriate levels of during biofilm formation in .

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2009-11-01
2024-03-28
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