1887

Abstract

The type III secretion system (T3SS) encoded by pathogenicity island 1 (SPI-1) is important for the invasion of epithelial cells during development of -associated enterocolitis. It has been suggested that the level and timing of the expression of the SPI-1 T3SS proteins and effectors dictate the consequences of bacterial infection and pathogenesis. However, the expression of these proteins has not been extensively studied , especially during the later stages of salmonellosis when the infection is established. We have constructed recombinant strains that contain a FLAG epitope inserted in-frame to genes , , , , and , and investigated the expression of the tagged proteins both and during murine salmonellosis. Mice were inoculated intraperitoneally or intragastrically with the tagged strains. At different time points post-infection, bacteria were recovered from various organs, and the expression of the tagged proteins was determined. Our results provide direct evidence that PrgJ and SipD are expressed in colonizing the liver and ileum of infected animals at both the early and late stages of infection. Furthermore, our study has shown that the InvJ protein is expressed preferentially in colonizing the ileum but not the liver, while SipC is expressed preferentially in colonizing the liver but not the ileum. Thus, appears to express different SPI-1 proteins and effectors when colonizing specific tissues. Our results suggest that differential expression of these proteins may be important for tissue-specific aspects of bacterial pathogenesis such as gastroenterititis in the ileum and systemic infection in the liver.

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2010-01-01
2019-08-26
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