1887

Abstract

In Gram-negative bacteria, autotransporter proteins constitute the largest family of secreted proteins, and exhibit many different functions. In recent years, research has largely focused on mechanisms of autotransporter protein translocation, where several alternative models are still being discussed. In contrast, the biogenesis of only a few autotransporters has been studied and, likewise, regulation of expression has received only very limited attention. The glycosylated autotransporter dhesin nvolved in iffuse dherence (AIDA)-I system consists of the gene, encoding a specific autotransporter adhesin heptosyltransferase (AAH), and the gene, encoding the autotransporter protein (AIDA-I). In this study, we investigated the promoter organization and transcription of these two genes using reporter plasmids carrying transcriptional fusions. The two genes, and , are transcribed as a bicistronic message. However, is additionally transcribed from its own promoter. There are two distinct start sites for each of the two genes. Interestingly, transcription of both genes is enhanced in and mutant backgrounds. Furthermore, we addressed the influence of environmental factors and different genetic backgrounds of K-12 strains on transcription activity. We found that transcription varied considerably in different K-12 laboratory strains and under different growth conditions.

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2010-04-01
2020-01-26
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