1887

Abstract

Virginiae butanolide (VB) is a -butyrolactone autoregulator that triggers production of the streptogramin antibiotic virginiamycin in . Our previous studies suggested that the gene, an -family gene, is likely to participate in the regulatory pathway for the production of VB, rather than in the biosynthetic pathway of VB itself, in contrast to the function of other -family genes. Mutation analysis now shows that BarX at least plays an enzymic role in the VB biosynthetic pathway. Heterologous expression of the gene from into the mutant partially restored the deficiency of virginiamycin production, suggesting that -family genes have a common ability to synthesize the -butyrolactone autoregulators. Taken together with previous works relating to the function of an -family gene, these results support the idea that streptomycetes have two biosynthetic pathways for the -butyrolactone autoregulators.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.032003-0
2010-01-01
2019-10-15
Loading full text...

Full text loading...

/deliver/fulltext/micro/156/1/206.html?itemId=/content/journal/micro/10.1099/mic.0.032003-0&mimeType=html&fmt=ahah

References

  1. Horinouchi, S. ( 2007; ). Mining and polishing of the treasure trove in the bacterial genus Streptomyces. Biosci Biotechnol Biochem 71, 283–299.[CrossRef]
    [Google Scholar]
  2. Hsiao, N. H., Soding, J., Linke, D., Lange, C., Hertweck, C., Wohlleben, W. & Takano, E. ( 2007; ). ScbA from Streptomyces coelicolor A3(2) has homology to fatty acid synthases and is able to synthesize γ-butyrolactones. Microbiology 153, 1394–1404.[CrossRef]
    [Google Scholar]
  3. Kato, J. Y., Funa, N., Watanabe, H., Ohnishi, Y. & Horinouchi, S. ( 2007; ). Biosynthesis of γ-butyrolactone autoregulators that switch on secondary metabolism and morphological development in Streptomyces. Proc Natl Acad Sci U S A 104, 2378–2383.[CrossRef]
    [Google Scholar]
  4. Kawachi, R., Akashi, T., Kamitani, Y., Sy, A., Wangchaisoonthorn, U., Nihira, T. & Yamada, Y. ( 2000; ). Identification of an AfsA homologue (BarX) from Streptomyces virginiae as a pleiotropic regulator controlling autoregulator biosynthesis, virginiamycin biosynthesis and virginiamycin M1 resistance. Mol Microbiol 36, 302–313.[CrossRef]
    [Google Scholar]
  5. Lee, Y. J., Kitani, S., Kinoshita, H. & Nihira, T. ( 2008; ). Identification by gene deletion analysis of barS2, a gene involved in the biosynthesis of γ-butyrolactone autoregulator in Streptomyces virginiae. Arch Microbiol 189, 367–374.[CrossRef]
    [Google Scholar]
  6. Nihira, T., Shimizu, Y., Kim, H. S. & Yamada, Y. ( 1988; ). Structure–activity relationships of virginiae butanolide C, an inducer of virginiamycin production in Streptomyces virginiae. J Antibiot (Tokyo) 41, 1828–1837.[CrossRef]
    [Google Scholar]
  7. Pulsawat, N., Kitani, S., Fukushima, E. & Nihira, T. ( 2009; ). Hierarchical control of virginiamycin production in Streptomyces virginiae by three pathway-specific regulators: VmsS, VmsT and VmsR. Microbiology 155, 1250–1259.[CrossRef]
    [Google Scholar]
  8. Sakuda, S., Higashi, A., Tanaka, S., Nihira, T. & Yamada, Y. ( 1992; ). Biosynthesis of virginiae butanolide A, a butyrolactone autoregulator from Streptomyces. J Am Chem Soc 114, 663–668.[CrossRef]
    [Google Scholar]
  9. Sakuda, S., Tanaka, S., Mizuno, K., Sukcharoen, O., Nihira, T. & Yamada, Y. ( 1993; ). Biosynthetic studies on virginiae butanolide A, a butyrolactone autoregulator from Streptomyces. Part 2. Preparation of possible biosynthetic intermediates and conversion experiments in a cell-free system. J Chem Soc Perkin Trans 1, 2309–2315.
    [Google Scholar]
  10. Shikura, N., Yamamura, J. & Nihira, T. ( 2002; ). barS1, a gene for biosynthesis of a γ-butyrolactone autoregulator, a microbial signaling molecule eliciting antibiotic production in Streptomyces species. J Bacteriol 184, 5151–5157.[CrossRef]
    [Google Scholar]
  11. Takano, E. ( 2006; ). γ-Butyrolactones: Streptomyces signalling molecules regulating antibiotic production and differentiation. Curr Opin Microbiol 9, 287–294.[CrossRef]
    [Google Scholar]
  12. Yamada, Y., Sugamura, K., Kondo, K., Yanagimoto, M. & Okada, H. ( 1987; ). The structure of inducing factors for virginiamycin production in Streptomyces virginiae. J Antibiot (Tokyo) 40, 496–504.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.032003-0
Loading
/content/journal/micro/10.1099/mic.0.032003-0
Loading

Data & Media loading...

Adobe PDF - mic032003_suppl_figure_legends.pdf 

PDF

Adobe PDF - mic032003_suppl_figures.pdf 

PDF

Adobe PDF - mic032003_suppl_tables.pdf 

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error