1887

Abstract

The gene for RNA helicase, CrhR, was one of the most highly induced genes when the cyanobacterium sp. PCC 6803 was exposed to a downward shift in ambient temperature. Although CrhR may be involved in the acclimatization of cyanobacterial cells to low-temperature environments, its functional role during the acclimatization is not known. In the present study, we mutated the gene by replacement with a spectinomycin-resistance gene cassette. The resultant Δ mutant exhibited a phenotype of slow growth at low temperatures. DNA microarray analysis of the genome-wide expression of genes, and Northern and Western blotting analyses indicated that mutation of the gene repressed the low-temperature-inducible expression of heat-shock genes and , at the transcript and protein levels. The kinetics of the co-transcript and the transcript after addition of rifampicin suggested that CrhR stabilized these transcripts at an early phase, namely 5–60 min, during acclimatization to low temperatures, and enhanced the transcription of these genes at a later time, namely 3–5 h. Our results suggest that CrhR regulates the low-temperature-inducible expression of these heat-shock proteins, which, in turn, may be essential for acclimatization of cells to low temperatures.

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2010-02-01
2019-12-11
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Strategy for disruption of the gene in the genome of sp. PCC 6803 [ PDF] (69 kb) DNA microarray analysis to compare the gene expression in Δ mutant cells with that in wild-type cells both grown at 34°C for 16 h [ PDF] (122 kb) DNA microarray analysis of the low temperature-induced expression of genes in wild-type and Δ mutant cells [ PDF] (418 kb) Levels of CrhR in wild-type, and Δc cells [ PDF] (62 kb) Northern blotting analysis of the heat-induced expression of the gene in wild-type and Δ cells [ PDF] (119 kb)

PDF

Strategy for disruption of the gene in the genome of sp. PCC 6803 [ PDF] (69 kb) DNA microarray analysis to compare the gene expression in Δ mutant cells with that in wild-type cells both grown at 34°C for 16 h [ PDF] (122 kb) DNA microarray analysis of the low temperature-induced expression of genes in wild-type and Δ mutant cells [ PDF] (418 kb) Levels of CrhR in wild-type, and Δc cells [ PDF] (62 kb) Northern blotting analysis of the heat-induced expression of the gene in wild-type and Δ cells [ PDF] (119 kb)

PDF

Strategy for disruption of the gene in the genome of sp. PCC 6803 [ PDF] (69 kb) DNA microarray analysis to compare the gene expression in Δ mutant cells with that in wild-type cells both grown at 34°C for 16 h [ PDF] (122 kb) DNA microarray analysis of the low temperature-induced expression of genes in wild-type and Δ mutant cells [ PDF] (418 kb) Levels of CrhR in wild-type, and Δc cells [ PDF] (62 kb) Northern blotting analysis of the heat-induced expression of the gene in wild-type and Δ cells [ PDF] (119 kb)

PDF

Strategy for disruption of the gene in the genome of sp. PCC 6803 [ PDF] (69 kb) DNA microarray analysis to compare the gene expression in Δ mutant cells with that in wild-type cells both grown at 34°C for 16 h [ PDF] (122 kb) DNA microarray analysis of the low temperature-induced expression of genes in wild-type and Δ mutant cells [ PDF] (418 kb) Levels of CrhR in wild-type, and Δc cells [ PDF] (62 kb) Northern blotting analysis of the heat-induced expression of the gene in wild-type and Δ cells [ PDF] (119 kb)

PDF

Strategy for disruption of the gene in the genome of sp. PCC 6803 [ PDF] (69 kb) DNA microarray analysis to compare the gene expression in Δ mutant cells with that in wild-type cells both grown at 34°C for 16 h [ PDF] (122 kb) DNA microarray analysis of the low temperature-induced expression of genes in wild-type and Δ mutant cells [ PDF] (418 kb) Levels of CrhR in wild-type, and Δc cells [ PDF] (62 kb) Northern blotting analysis of the heat-induced expression of the gene in wild-type and Δ cells [ PDF] (119 kb)

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