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Volume 155, Issue 11

Influence of a model human defensive peroxidase system on oral streptococcal antagonism Free

Abstract

is a dominant genus in the human oral cavity, making up about 20 % of the more than 800 species of bacteria that have been identified, and about 80 % of the early biofilm colonizers. Oral streptococci include both health-compatible (e.g. and ) and pathogenic strains (e.g. the cariogenic ). Because the streptococci have similar metabolic requirements, they have developed defence strategies that lead to antagonism (also known as bacterial interference). expresses bacteriocins that are cytotoxic toward and , whereas and differentially produce HO (under aerobic growth conditions), which is relatively toxic toward . Superimposed on the inter-bacterial combat are the effects of the host defensive mechanisms. We report here on the multifarious effects of bovine lactoperoxidase (bLPO) on the antagonism between and versus . Some of the effects are apparently counterproductive with respect to maintaining a health-compatible population of streptococci. For example, the bLPO system (comprised of bLPO+SCN+HO) destroys HO, thereby abolishing the ability of and to inhibit the growth of . Furthermore, bLPO protein (with or without its substrate) inhibits bacterial growth in a biofilm assay, but sucrose negates the inhibitory effects of the bLPO protein, thereby facilitating adherence of in lieu of and . Our findings may be relevant to environmental pressures that select early supragingival colonizers.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): bLPO, bovine milk lactoperoxidase , CLSM, confocal laser scanning microscopy , CV, crystal violet , DTNB, 5,5′-dithio-bis(2-nitrobenzoic acid) , hMPO, human myeloperoxidase , HRP, horseradish peroxidase , hSPO, human salivary peroxidase and LPO, lactoperoxidase
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2009-11-01
2024-04-19
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Influence of a model human defensive peroxidase system on oral streptococcal antagonism
Microbiology 155, 3691 (2009); https://doi.org/10.1099/mic.0.031310-0
/content/journal/micro/10.1099/mic.0.031310-0
/content/journal/micro/10.1099/mic.0.031310-0
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