1887

Abstract

(formerly PA2528-PA2527-PA2526-), encoding a putative resistance nodulation cell division (RND)-type multidrug efflux pump system, was cloned from PAO1. Introduction of into YM64, a drug-hypersusceptible strain, led to elevated MICs of aztreonam, macrolides, novobiocin and tetracycline. Since and , both of which encode RND components, were essential for function, MuxABC-OpmB is thought to be a drug efflux pump with four components. One novobiocin-resistant mutant, PMX725, isolated from PMX7 showed elevated resistance not only to novobiocin but also to aztreonam, macrolides and tetracycline. Increased mRNA expression of was observed in the mutant PMX725 compared with the parental strain. Sequencing analysis revealed that a single-nucleotide insertion had occurred in the deduced promoter region for in PMX725. In this study, we have characterized the last RND-type multidrug efflux pump predicted from the genome sequence in .

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2009-11-01
2020-08-15
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