1887

Abstract

is a ubiquitous bacterium capable of infecting humans, particularly pregnant women and immunocompromised individuals. Although the intracellular invasion and pathogenesis of listeriosis in mammalian tissues has been well studied, little is known about the ecology of , and in particular the environmental reservoir for this bacterium has not been identified. This study used short-term co-culture at 15, 22 and 37 °C to examine the interaction of strains with ACO12. Survival of cells phagocytosed by monolayers of trophozoites was assessed by culture techniques and microscopy. trophozoites eliminated bacterial cells within a few hours post-phagocytosis, irrespective of the incubation temperature used. Wild-type and a phenotypic listeriolysin O mutant were unable to either multiply or survive within trophozoites. By contrast, serovar Typhimurium C5 cells used as controls were able to survive and multiply within trophozoites. The data presented indicate that ACO12 is unlikely to harbour , or act as an environmental reservoir for this bacterium.

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2010-03-01
2020-02-20
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References

  1. Akya A., Thomas C. J.. 2009; Viability of Listeria monocytogenes in co-culture with Acanthamoeba spp. FEMS Microbiol Ecol70:20–29
    [Google Scholar]
  2. Allerberger F.. 2003; Listeria: growth, phenotypic differentiation and molecular microbiology. FEMS Immunol Med Microbiol35:183–189
    [Google Scholar]
  3. Axelsson-Olsson D., Waldenström J., Broman T., Olsen B., Holmberg M.. 2005; Protozoan Acanthamoeba polyphaga as a potential reservoir for Campylobacter jejuni. Appl Environ Microbiol71:987–992
    [Google Scholar]
  4. Bozue J. A., Johnson W.. 1996; Interaction of Legionella pneumophila with Acanthamoeba castellanii: uptake by coiling phagocytosis and inhibition of phagosome–lysosome fusion. Infect Immun64:668–673
    [Google Scholar]
  5. Brandl M. T., Rosenthal B. M., Haxo A. F., Berk S. G.. 2005; Enhanced survival of Salmonella enterica in vesicles released by a soilborne Tetrahymena species. Appl Environ Microbiol71:1562–1569
    [Google Scholar]
  6. Cirillo J. D.. 1999; Exploring a novel perspective on pathogenic relationships. Trends Microbiol7:96–98
    [Google Scholar]
  7. Cirillo J. D., Falkow S., Tompkins L. S.. 1994; Growth of Legionella pneumophila in Acanthamoeba castellanii enhances invasion. Infect Immun62:3254–3326
    [Google Scholar]
  8. Cirillo J. D., Falkow S., Tompkins L. S., Bermudez L. E.. 1997; Interaction of Mycobacterium avium with environmental amoebae enhances virulence. Infect Immun65:3759–3767
    [Google Scholar]
  9. Cossart P.. 2002; Molecular and cellular basis of the infection by Listeria monocytogenes: an overview. Int J Med Microbiol291:401–409
    [Google Scholar]
  10. Cossart P., Sansonetti P. J.. 2004; Bacterial invasion: the paradigms of enteroinvasive pathogens. Science304:242–248
    [Google Scholar]
  11. Daggett P.-M., Sawyer T. K., Nerad T. A.. 1982; Distribution and possible interrelationships of pathogenic and non-pathogenic Acanthamoeba from aquatic environments. Microb Ecol8:371–386
    [Google Scholar]
  12. Francis M. S., Thomas C. J.. 1996; Effect of multiplicity of infection on Listeria monocytogenes pathogenicity for HeLa and Caco-2 cell lines. J Med Microbiol45:323–330
    [Google Scholar]
  13. Gao L. Y., Harb O. S., Abu Kwaik Y.. 1997; Utilization of similar mechanisms by Legionella pneumophila to parasitize two evolutionarily distant host cells, mammalian macrophages and protozoa. Infect Immun65:4738–4746
    [Google Scholar]
  14. Gaze W. H., Burroughs N., Gallagher M. P., Wellington E. M.. 2003; Interactions between Salmonella typhimurium and Acanthamoeba polyphaga, and observation of a new mode of intracellular growth within contractile vacuoles. Microb Ecol46:358–369
    [Google Scholar]
  15. Greub G., Raoult D.. 2002; Crescent bodies of Parachlamydia acanthamoeba and its life cycle within Acanthamoeba polyphaga: an electron micrograph study. Appl Environ Microbiol68:3076–3084
    [Google Scholar]
  16. Greub G., Raoult D.. 2004; Microorganisms resistant to free-living amoebae. Clin Microbiol Rev17:413–433
    [Google Scholar]
  17. Harb O. S., Gao L. Y., Abu Kwaik Y.. 2000; From protozoa to mammalian cells: a new paradigm in the life cycle of intracellular bacterial pathogens. Environ Microbiol2:251–265
    [Google Scholar]
  18. Herd M., Kocks C.. 2001; Gene fragments distinguishing an epidemic-associated strain from a virulent prototype strain of Listeria monocytogenes belong to distinct functional subset of genes and partially cross-hybridize with other Listeria species. Infect Immun69:3972–3979
    [Google Scholar]
  19. Huws S. A., Morley R. J., Jones M. V., Brown M. R., Smith A. W.. 2008; Interactions of some common pathogenic bacteria with Acanthamoeba polyphaga. FEMS Microbiol Lett282:258–265
    [Google Scholar]
  20. Kahane S., Dvoskin B., Mathias M., Friedman M. G.. 2001; Infection of Acanthamoeba polyphaga with Simkania negevensis and S. negevensis survival within amoebal cysts. Appl Environ Microbiol67:4789–4795
    [Google Scholar]
  21. Lamothe J., Thyssen S., Valvano M. A.. 2004; Burkholderia cepacia complex isolates survive intracellularly without replication within acidic vacuoles of Acanthamoeba polyphaga. Cell Microbiol6:1127–1138
    [Google Scholar]
  22. Landers P., Kerr K. G., Rowbotham T. J., Tipper J. L., Keig P. M., Ingham E., Denton M.. 2000; Survival and growth of Burkholderia cepacia within the free-living amoeba Acanthamoeba polyphaga. Eur J Clin Microbiol Infect Dis19:121–123
    [Google Scholar]
  23. Ly T. M., Müller H. E.. 1990; Ingested Listeria monocytogenes survive and multiply in protozoa. J Med Microbiol33:51–54
    [Google Scholar]
  24. Marolda C. L., Hauroder B., John M. A., Michel R., Valvano M. A.. 1999; Intracellular survival and saprophytic growth of isolates from the Burkholderia cepacia complex in free-living amoebae. Microbiology145:1509–1517
    [Google Scholar]
  25. Matz C., Kjelleberg S.. 2005; Off the hook – how bacteria survive protozoan grazing. Trends Microbiol13:302–307
    [Google Scholar]
  26. Molmeret M., Horn M., Wagner M., Santic M., Abu Kwaik Y.. 2005; Amoebae as training grounds for intracellular bacterial pathogens. Appl Environ Microbiol71:20–28
    [Google Scholar]
  27. Newsome A. L., Scott T. M., Benson R. F., Fields B. S.. 1998; Isolation of an amoeba naturally harboring a distinctive Legionella species. Appl Environ Microbiol64:1688–1693
    [Google Scholar]
  28. Roberts A. J., Wiedmann M.. 2003; Pathogen, host and environmental factors contributing to the pathogenesis of listeriosis. Cell Mol Life Sci60:904–918
    [Google Scholar]
  29. Rowbotham T. J.. 1983; Isolation of Legionella pneumophila from clinical specimens via amoebae, and the interaction of those and other isolates with amoebae. J Clin Pathol36:978–986
    [Google Scholar]
  30. Segal G., Shuman H. A.. 1999; Legionella pneumophila utilizes the same genes to multiply within Acanthamoeba castellanii and human macrophages. Infect Immun67:2117–2124
    [Google Scholar]
  31. Smirnov A. V., Brown S.. 2004; Guide to the methods of study and identification of soil gymnamoebae. Protistology3:148–190
    [Google Scholar]
  32. Steinert M., Birkness K., White E., Fields B., Quinn F.. 1998; Mycobacterium avium bacilli grow saprozoically in coculture with Acanthamoeba polyphaga and survive within cyst walls. Appl Environ Microbiol64:2256–2261
    [Google Scholar]
  33. Taylor S. J., Ahonen L. J., de Leij F. A., Dale J. W.. 2003; Infection of Acanthamoeba castellanii with Mycobacterium bovis and M. bovis BCG and survival of M. bovis within the amoebae. Appl Environ Microbiol69:4316–4319
    [Google Scholar]
  34. Thom S., Warhurst D., Drasar B. S.. 1992; Association of Vibrio cholerae with fresh water amoebae. J Med Microbiol36:303–306
    [Google Scholar]
  35. Vázquez-Boland J. A., Kuhn M., Berche P., Chakraborty T., Dominguez-Bernal G., Goebel W., González-Zorn B., Wehland J., Kreft J.. 2001; Listeria pathogenesis and molecular virulence determinants. Clin Microbiol Rev14:584–640
    [Google Scholar]
  36. Wing E. J., Gregory S. H.. 2002; Listeria monocytogenes: clinical and experimental update. J Infect Dis185:Suppl. 1S18–S24
    [Google Scholar]
  37. Winiecka-Krusnell J., Linder E.. 1999; Free-living amoebae protecting Legionella in water: the tip of an iceberg?. Scand J Infect Dis31:383–385
    [Google Scholar]
  38. Zhou X., Elmose J., Call D. R.. 2007; Interactions between the environmental pathogen Listeria monocytogenes and a free-living protozoan ( Acanthamoeba castellanii. Environ Microbiol9:913–922
    [Google Scholar]
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