1887

Abstract

In addition to its known status as a disseminated disease in HIV-positive patients, complex (MAC) is increasingly recognized as a causative pathogen of respiratory disease in HIV-negative patients. MAC is divided into , and the less-epidemiologically studied . Genetic typing for using variable number of tandem repeats (VNTR) has not yet been developed. The aim of this study was to identify VNTR loci in the genome of and apply them as an epidemiological tool to clinical isolates. Here, we identified 25 VNTR loci on the genome, of which 16 showed variations among clinical isolates in the number of tandem repeat motifs. Among the 74 isolates, 50 genotypes were distinguished using the 16 VNTR loci, resulting in a Hunter Gaston's discriminatory index of 0.988. Moreover, all 16 VNTR loci were stable in different sets of isolates recovered within time intervals ranging from 2 to 1551 days from 14 separate patients. These results indicate that for use as epidemiological markers of , the loci in this VNTR assay are highly discriminating and stable over time.

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2010-02-01
2019-11-22
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IS sequence obtained from the sequencing of clinical isolates in this study. Arrows indicate tandem repeat units and the blue box indicates the insertion sequence IS . The IS sequence was provided by P. Siguier from the IS database registry ( http://www-is.biotoul.fr). [ PDF] (159 kb) PCR amplification products of the tandem repeat loci analysed by agarose gel electrophoresis. [ PDF] (83 kb) Repeat polymorphisms in VNTR loci aligned from sequenced PCR products of ATCC 13950 and clinical strains. The sequences of the repeat regions and flanking DNA are shown for the 16 variable loci. A dash represents deletion of a repeat. Arrows indicate tandem repeat (TR) units. [ PDF] (345 kb)

PDF

IS sequence obtained from the sequencing of clinical isolates in this study. Arrows indicate tandem repeat units and the blue box indicates the insertion sequence IS . The IS sequence was provided by P. Siguier from the IS database registry ( http://www-is.biotoul.fr). [ PDF] (159 kb) PCR amplification products of the tandem repeat loci analysed by agarose gel electrophoresis. [ PDF] (83 kb) Repeat polymorphisms in VNTR loci aligned from sequenced PCR products of ATCC 13950 and clinical strains. The sequences of the repeat regions and flanking DNA are shown for the 16 variable loci. A dash represents deletion of a repeat. Arrows indicate tandem repeat (TR) units. [ PDF] (345 kb)

PDF

IS sequence obtained from the sequencing of clinical isolates in this study. Arrows indicate tandem repeat units and the blue box indicates the insertion sequence IS . The IS sequence was provided by P. Siguier from the IS database registry ( http://www-is.biotoul.fr). [ PDF] (159 kb) PCR amplification products of the tandem repeat loci analysed by agarose gel electrophoresis. [ PDF] (83 kb) Repeat polymorphisms in VNTR loci aligned from sequenced PCR products of ATCC 13950 and clinical strains. The sequences of the repeat regions and flanking DNA are shown for the 16 variable loci. A dash represents deletion of a repeat. Arrows indicate tandem repeat (TR) units. [ PDF] (345 kb)

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