1887

Abstract

The enzyme phosphoglucomutase (Pgm) catalyses the interconversion of glucose 1-phosphate and glucose 6-phosphate and contributes to glycolysis and the generation of sugar nucleotides for biosynthesis. To assess the role of this enzyme in the biology of the pathogen serovar Typhimurium we have characterized a deletion mutant in strain SL1344. Compared to SL1344, SL1344 had impaired growth , was deficient in the ability to utilize galactose as a carbon source and displayed reduced O-antigen polymer length. The mutant was also more susceptible to antimicrobial peptides and showed decreased fitness in the mouse typhoid model. The phenotype of SL1344 indicated a role for in the early stages of infection, most likely through deficient O-antigen production. Although mutants in other pathogens have potential as live attenuated vaccine strains, SL1344 was not sufficiently attenuated for such use.

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2009-10-01
2019-10-15
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