1887

Abstract

The virulence of four isolates was compared in a murine model of sporotrichosis, together with the protein pattern of the yeast cell surface and the capacity to bind the extracellular matrix protein fibronectin. Virulence was determined by the mortality rate, fungal burden and histopathology. Two clinical isolates were more virulent for C57BL/6 mice, but no direct correlation was seen between virulence and the clinical or environmental origin of the isolates. The lowest virulence was observed for an isolate recovered from a patient with meningeal sporotrichosis. Although all isolates could effectively disseminate, the dissemination patterns were not similar. Using flow cytometry analysis, we investigated the interaction of all the strains with fibronectin, and showed that the binding capacity correlated with virulence. Western blot analysis of cell wall extracts revealed positive bands for fibronectin in the range of 37–92 kDa. The 70 kDa adhesin was also recognized by a protective monoclonal antibody raised against a gp70 antigen of (mAb P6E7). Confocal microscopy confirmed the co-localization of fibronectin and mAb P6E7 on the yeast cell surface. To our knowledge, this is the first report identifying adhesins for fibronectin on the surface of this human pathogen.

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2009-11-01
2020-07-14
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References

  1. Albrecht A., Felk A., Pichova I., Naglik J. R., Schaller M., De Groot P., MacCallum D., Odds F. C., Schafer W.. other authors 2006; Glycosylphosphatidylinositol-anchored proteases of Candida albicans target proteins necessary for both cellular processes and host-pathogen interactions. J Biol Chem281:688–694
    [Google Scholar]
  2. Bjellqvist B., Pasquali C., Ravier F., Sanchez J. C., Hochstrasser D.. 1993; A nonlinear wide-range immobilized pH gradient for two-dimensional electrophoresis and its definition in a relevant pH scale. Electrophoresis14:1357–1365
    [Google Scholar]
  3. Brandhorst T. T., Wuthrich M., Warner T., Klein B.. 1999; Targeted gene disruption reveals an adhesin indispensable for pathogenicity of Blastomyces dermatitidis. J Exp Med189:1207–1216
    [Google Scholar]
  4. Fernandes K. S., Coelho A. L., Lopes Bezerra L. M., Barja-Fidalgo C.. 2000; Virulence of Sporothrix schenckii conidia and yeast cells, and their susceptibility to nitric oxide. Immunology101:563–569
    [Google Scholar]
  5. Fernandes K. S., Neto E. H., Brito M. M., Silva J. S., Cunha F. Q., Barja-Fidalgo C.. 2008; Detrimental role of endogenous nitric oxide in host defence against Sporothrix schenckii. Immunology123:469–479
    [Google Scholar]
  6. Figueiredo C. C., Deccache P. M., Lopes-Bezerra L. M., Morandi V.. 2007; TGF- β1 induces transendothelial migration of the pathogenic fungus Sporothrix schenckii by a paracellular route involving extracellular matrix proteins. Microbiology153:2910–2921
    [Google Scholar]
  7. Grocott R. G.. 1955; A stain for fungi in tissue sections and smears using Gomori's methenamine-silver nitrate technique. Am J Clin Pathol25:975–979
    [Google Scholar]
  8. Hanna S. A., Monteiro da Silva J. L., Mendes-Giannini M. J. S.. 2000; Adherence and intracellular parasitism of Paracoccidioides brasiliensis in Vero cells. Microbes Infect2:877–884
    [Google Scholar]
  9. Idnurm A., Bahn Y. S., Nielsen K., Lin X., Fraser J. A., Heitman J.. 2005; Deciphering the model pathogenic fungus Cryptococcus neoformans. Nat Rev Microbiol3:753–764
    [Google Scholar]
  10. Klis F. M., de Jong M., Brul S., de Groot P. W.. 2007; Extraction of cell surface-associated proteins from living yeast cells. Yeast24:253–258
    [Google Scholar]
  11. Köhler G., Milstein C.. 1975; Continuous cultures of fused cells secreting antibody of redefined specificity. Nature256:495–497
    [Google Scholar]
  12. Kong X., Xiao T., Lin J., Wang Y., Chen H. D.. 2006; Relationships among genotypes, virulence and clinical forms of Sporothrix schenckii infection. Clin Microbiol Infect12:1077–1081
    [Google Scholar]
  13. Laemmli U. K.. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature227:680–685
    [Google Scholar]
  14. Lima O. C., Figueiredo C. C., Pereira B. A., Coelho M. G., Morandi V., Lopes-Bezerra L. M.. 1999; Adhesion of the human pathogen Sporothrix schenckii to several extracellular matrix proteins. Braz J Med Biol Res32:651–657
    [Google Scholar]
  15. Lima O. C., Figueiredo C. C., Previato J. O., Mendonça-Previato L., Morandi V., Lopes-Bezerra L. M.. 2001; Involvement of fungal cell wall components in adhesion of Sporothrix schenckii to human fibronectin. Infect Immun69:6874–6880
    [Google Scholar]
  16. Lima O. C., Bouchara J. P., Renier G., Marot-Leblond A., Chabasse D., Lopes-Bezerra L. M.. 2004; Immunofluorescence and flow cytometry analysis of fibronectin and laminin binding to Sporothrix schenckii yeast cells and conidia. Microb Pathog37:131–140
    [Google Scholar]
  17. Lopes-Bezerra L. M., Schubach A., Costa O. R.. 2006; Sporothrix schenckii and sporotrichosis. An Acad Bras cienc78:293–308
    [Google Scholar]
  18. Maia D. C., Sassa M. F., Placeres M. C., Carlos I. Z.. 2006; Influence of Th1/Th2 cytokines and nitric oxide in murine systemic infection induced by Sporothrix schenckii. Mycopathologia161:11–19
    [Google Scholar]
  19. Marimon R., Gené J., Cano J., Trilles L., Dos Santos Lazéra M., Guarro J.. 2006; Molecular phylogeny of Sporothrix schenckii. J Clin Microbiol44:3251–3256
    [Google Scholar]
  20. Mendes-Giannini M. J., Andreotti P. F., Vincenzi L. R., da Silva J. L., Lenzi H. L., Benard G., Zancope-Oliveira R., de Matos Guedes H. L., Soares C. P.. 2006; Binding of extracellular matrix proteins to Paracoccidioides brasiliensis. Microbes Infect8:1550–1559
    [Google Scholar]
  21. Nascimento R. C., Espíndola N. M., Castro R. A., Teixeira P. A., Loureiro y Penha C. V., Lopes-Bezerra L. M., Almeida S. R.. 2008; Passive immunization with monoclonal antibody against a 70-kDa putative adhesin of Sporothrix schenckii induces protection in murine sporotrichosis. Eur J Immunol38:3080–3089
    [Google Scholar]
  22. Patti J. M., Allen B. L., McGavin M. J., Hook M.. 1994; MSCRAMM-mediated adherence of microorganisms to host tissues. Annu Rev Microbiol48:585–617
    [Google Scholar]
  23. Pirofski L. A., Casadevall A.. 2008; The damage-response framework of microbial pathogenesis and infectious diseases. Adv Exp Med Biol635:135–146
    [Google Scholar]
  24. Prophet E. B., Mills B., Arrington J. B., Sobin L. H.. 1992; Laboratory Methods in Histotechnology, Chapter 18: Carbohydrates Washington DC: Armed Forces Institute of Pathology, American Registry of Pathology;
    [Google Scholar]
  25. Rosas A. L., Nosanchuk J. D., Casadevall A.. 2001; Passive immunization with melanin-binding monoclonal antibodies prolongs survival of mice with lethal Cryptococcus neoformans infection. Infect Immun69:3410–3412
    [Google Scholar]
  26. Ruiz-Baca E., Toriello C., Pérez-Torres A., Sabanero-López M., Villagómez-Castro J. C., López-Romero E.. 2008; Isolation and some properties of a glycoprotein of 70 kDa (Gp70) from the cell wall of Sporothrix schenckii involved in fungal adherence to dermal extracellular matrix. Med Mycol47:185–196
    [Google Scholar]
  27. Tachibana T., Matsuyama T., Mitsuyama M.. 1998; Characteristic infectivity of Sporothrix schenckii to mice depending on routes of infection and inherent fungal pathogenicity. Med Mycol36:21–27
    [Google Scholar]
  28. Tachibana T., Matsuyama T., Ito M., Mitsuyama M.. 2001; Sporothrix schenckii thermo-intolerant mutants losing fatal visceral infectivity but retaining high cutaneous infectivity. Med Mycol39:295–298
    [Google Scholar]
  29. Wasylnka J. A., Moore M. M.. 2000; Adhesion of Aspergillus species to extracellular matrix proteins: evidence for involvement of negatively charged carbohydrates on the conidial surface. Infect Immun68:3377–3384
    [Google Scholar]
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