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Abstract

To clarify the budding pattern of , the vegetative cells were observed by scanning electron microscopy. The cells grew by bipolar budding, but cells that budded from the shoulder of a mother cell were occasionally observed. We examined the cell morphology and phylogeny of five strains of sp. isolated in Thailand as well as seven and three sp. strains that were preserved in culture collections. Phylogenetic analysis based on three different nucleotide sequences (D1/D2 domain of 26S rDNA, the actin gene and the elongation factor 2 gene ) indicated that all the strains belonged to the genus and were neighbours of the type strain NBRC 1290. The strains fell into two groups in this analysis. The budding patterns of the strains were carefully observed by staining the bud scars, and these patterns were categorized into three groups: types I–III. Type I included cells that grew by bipolar budding and formed multiple scars, type III included cells that grew by multilateral budding and formed a single scar, and type II included cells that exhibited a mixture of type I and type III patterns. Among the 15 strains, 12 strains, including NBRC 1290, mainly exhibited type I or type II budding patterns; these strains belonged to group 1 of the phylogenetic analysis. The remaining three strains, which belonged to group 2, exhibited either type II or type III patterns. Thus the phylogenetic relationship and budding patterns are related. Moreover, some cells also exhibited budding characteristics that were intermediate between bipolar and multilateral budding.

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2009-09-01
2019-10-16
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References

  1. Diezmann, S., Cox, C. J., Schönian, G., Vilgalys, R. J. & Mitchell, T. G. ( 2004; ). Phylogeny and evolution of medical species of Candida and related taxa: a multigenic analysis. J Clin Microbiol 42, 5624–5635.[CrossRef]
    [Google Scholar]
  2. Ditlevsen, E. & Hjort, A. ( 1964; ). Hanseniaspora pijperi (van der Walt et Tscheuschner) nov. comb. C R Trav Lab Carlsberg 34, 347–357.
    [Google Scholar]
  3. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  4. Harkins, H. A., Page, N., Schenkman, L. R., Virgilio, C. D., Shaw, S., Bussey, H. & Pringle, J. R. ( 2001; ). Bud8p and Bud9p, proteins that may mark the sites for bipolar budding in yeast. Mol Biol Cell 12, 2497–2518.[CrossRef]
    [Google Scholar]
  5. Imanishi, Y., Ueda-Nishimura, K. & Mikata, K. ( 2007; ). Two new species of Kazachstania that form ascospores connected by a belt-like intersporal body: Kazachstania zonata and Kazachstania gamospora. FEMS Yeast Res 7, 330–338.[CrossRef]
    [Google Scholar]
  6. Jindamorakot, S., Am-in, S., Thuy, T. T., Duy, N. D., Kawasaki, H., Potacharoen, W., Limtong, S., Tanticharoen, M. & Nakase, T. ( 2004; ). Candida easanensis sp. nov., Candida pattaniensis sp. nov. and Candida nakhonratchasimensis sp. nov., three new species of yeasts isolated from insect frass in Thailand. J Gen Appl Microbiol 50, 261–269.[CrossRef]
    [Google Scholar]
  7. Kreger-van Rij, N. J. W. ( 1984; ). Pichia Hansen. In The Yeasts, a Taxonomic Study, 2nd revised and enlarged edn, pp. 455–554. Edited by J. Lodder. Amsterdam: North-Holland Publishing.
  8. Kurtzman, C. P. ( 1998; ). Discussion of teleomorphic and anamorphic ascomycetous yeasts and a key to genera. In The Yeasts, a Taxonomic Study, 4th edn, pp. 111–121. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
  9. Kurtzman, C. P. ( 1998; ). Pichia E.C. Hansen emend. Kurtzman. In The Yeasts, a Taxonomic Study, 4th edn, pp. 273–352. Edited by C. P. Kurtzman & J. W. Fell. Amsterdam: Elsevier.
  10. Kurtzman, C. P. & Robnett, C. J. ( 1997; ). Identification of clinically important ascomycetous yeasts based on nucleotide divergence in the 5′ end of the large-subunit (26S) ribosomal DNA gene. J Clin Microbiol 35, 1216–1223.
    [Google Scholar]
  11. Kurtzman, C. P., Robnett, C. J. & Basehoar-Powers, E. ( 2008; ). Phylogenetic relationships among species of Pichia, Issatchenkia and Williospsis determined from multigene sequence analysis, and the proposal of Barnettozyma gen. nov., Lindnera gen. nov. and Wickerhamomyces gen. nov. FEMS Yeast Res 8, 939–954.[CrossRef]
    [Google Scholar]
  12. Mikata, K. & Nakase, T. ( 1997; ). Surface structure of ascospores of genus Nadsonia Sydow. Microbiol Cult Collect 13, 97–102.
    [Google Scholar]
  13. Nanninga, N. ( 2001; ). Cytokinesis in prokaryotes and eukaryotes: common principles and different solutions. Microbiol Mol Biol Rev 65, 319–333.[CrossRef]
    [Google Scholar]
  14. Ni, L. & Snyder, M. ( 2001; ). A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae. Mol Biol Cell 12, 2147–2170.[CrossRef]
    [Google Scholar]
  15. Powell, C. D., Quain, D. E. & Smart, K. A. ( 2003; ). Chitin scar breaks in aged Saccharomyces cerevisiae. Microbiology 149, 3129–3137.[CrossRef]
    [Google Scholar]
  16. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  17. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w improving the sensitivity of progressive multiple sequence alignment through sequence weighting position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  18. Tsai, P. C., Lee, S. W., Liu, Y. W., Chu, C. W., Chen, K. Y., Ho, J. C. & Lee, F. J. S. ( 2008; ). Afi1p functions as an Arf3p polarization-specific docking factor for development of polarity. J Biol Chem 283, 16915–16927.[CrossRef]
    [Google Scholar]
  19. van der Walt, J. P. & Tscheuschner, I. T. ( 1957; ). Three new yeasts. Antonie Van Leeuwenhoek 23, 184–190.[CrossRef]
    [Google Scholar]
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vol. , part 9, pp. 3142 - 3148

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