1887

Abstract

pv. () causes bacterial blight disease in rice, one of the most serious rice diseases. The gene from strain 13751 encodes a protein containing a histone-like nucleoid-structuring protein (H-NS) domain. The expression of in strain 13751 was enhanced in XOM2 minimal medium. Mutation of the gene of strain 13751 led to a significant reduction in virulence in the host plant rice, a delayed hypersensitive response in the nonhost castor-oil plant, a decrease in extracellular polysaccharide and diffusible signal factor production, and an increase in intracellular glycogen accumulation. Northern hybridization analyses revealed that the virulence-associated genes , , , , and were downregulated in the mutant compared to the wild-type and complemented strains. Interestingly, increase of copy number of in the wild-type strain 13751 resulted in a strain showing similar phenotypes as the mutant and a reduction of the expression of , , , and . These findings indicate that the gene, which is highly conserved in the sequenced strains of , encodes an important regulatory factor for the virulence of .

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2009-09-01
2020-01-17
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