1887

Abstract

The GinI/GinR quorum-sensing system represses oxidative fermentation, including acetic acid and gluconic acid fermentation, as well as antifoam activity in NCI1051. An 89 aa protein, GinA, whose production is induced by the quorum-sensing system, represses both oxidative fermentation and antifoam activity via a still unknown mechanism, although an OmpA family protein, GmpA, as a target of the GinI/GinR quorum-sensing system via GinA, has been found to repress oxidative fermentation. In this study, four novel GinA-inducible genes (, , and ) were identified and their involvement in oxidative fermentation and antifoam activity was examined by gene disruption. Disruption of (which encodes a putative -acetylglucosamine-6-phosphate deacetylase) decreased the growth rate in the exponential growth phase, indicating that was required for the rapid growth of the strain. This unexpected finding revealed a new aspect of the GinI/GinR quorum-sensing system: it accelerates exponential growth by induction of . In contrast, (a putative glycosyltransferase) and (a putative cyclic-di-GMP phosphodiesterase) were shown to repress oxidative fermentation, including acetic acid and gluconic acid fermentation. was also shown to repress antifoam activity. Disruption of (a putative phosphodiesterase/diguanylate cyclase) caused no phenotypic changes. Taking our previous results into consideration, these results showed an apparently complex mechanism for repressing oxidative fermentation by the quorum-sensing system; at least three GinA-inducible genes, , and , were involved in the repression of oxidative fermentation by the GinI/GinR quorum-sensing system, the most characteristic feature of the acetic acid bacteria.

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2009-09-01
2019-12-12
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