1887

Abstract

The sensor kinase AbsA1 (SCO3225) phosphorylates the response regulator AbsA2 (SCO3226) and dephosphorylates AbsA2∼P. The phosphorylated response regulator represses antibiotic biosynthesis operons in . AbsA1 was predicted to have an atypical transmembrane topology, and the location of its signal-sensing domain is not readily obvious. To better understand this protein and to gain insight into its signal response mechanism, we determined its transmembrane topology using fusions of to , which is believed to be the first application of this approach to transmembrane topology in the actinomycetes. Our results are in agreement with the topological predictions and demonstrate that AbsA1 has five transmembrane domains, four near the N terminus and one near the C terminus. Unlike most sensor kinases, the largest extracellular portion of AbsA1 is at the C terminus.

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2009-06-01
2019-12-09
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