1887

Abstract

is an enteric bacterium which must overcome the acidic stress in host organs for successful colonization, but how this bacterium survives in acidic conditions remains largely unknown. In the present study, the importance of OmpR in acid survival of YpIII was confirmed by the fact that mutation of (strain Δ) greatly reduced cell survival at pH 4.5 or lower. To characterize the regulatory role of OmpR in this acid survival process, proteomic analysis was carried out to compare YpIII at pH 7.0 and pH 4.5 with Δ at pH 7.0, and urease components were revealed to be the main targets for OmpR regulation. Addition of urea to the culture medium also enhanced acid survival of YpIII but not Δ and urease activity was significantly induced by acid in YpIII but not in Δ. Each of the seven components of the YpIII urease gene cluster was fused to a reporter and their expression was dramatically decreased in a Δ background; this supports the notion that OmpR positively regulates urease expression. Furthermore, gel shift analysis revealed that OmpR binds to the deduced promoter regions of three polycistronic transcriptional units (, and ) in the urease cluster, suggesting that the regulation of OmpR to urease components is direct. Taken together, these data strongly suggest that OmpR activates urease expression to enhance acid survival in .

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2009-08-01
2019-10-20
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vol. , part 8, pp. 2522 - 2531

Supplementary material [ PDF, 153 kb], including: Primers used in this study OmpR regulated proteins related to acid response in YpIII Effect of ATR (pH 4.5) on AR (pH 3.0) in YpIII, Δ and Δ in the presence of urea Interaction of non-phosphorylated OmpR with the Fa fragment in a gel-retardation assay



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