1887

Abstract

Pulsed-field gel electrophoresis analysis of chromosomal single and double restriction profiles of 17 strains belonging to three genera of ‘’ was done, resulting in physical and genetic maps for three , six and four strains. I, I-I, I and I restriction enzymes were used together with Southern hybridization of selected probes to provide an assessment of genomic organization in different species. Estimated genome sizes varied from 1408 kb to 1547 kb in , from 1644 kb to 2133 kb in and from 1371 kb to 2197 kb in . Other genomic characteristics of interest were analysed, such as and localization and operon organization. The latter seems markedly different in , in both number and disposition in the chromosome. Comparisons of intra- and intergeneric features are discussed in the light of chromosome rearrangements and genomic evolution.

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2010-02-01
2019-10-17
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(see text of main paper for details): ( subsp. CECT 872 ) [ PDF] (126 kb) ( LC51 ) [ PDF] (241 kb) ( DSM 7378 [ PDF] (411 kb) PCR conditions used to amplify DNA sequences used as probes [ PDF] (24 kb) Physical and genetic map comparison of subsp. CECT 219T and KM20 sequenced strains [ PDF] (35 kb)

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(see text of main paper for details): ( subsp. CECT 872 ) [ PDF] (126 kb) ( LC51 ) [ PDF] (241 kb) ( DSM 7378 [ PDF] (411 kb) PCR conditions used to amplify DNA sequences used as probes [ PDF] (24 kb) Physical and genetic map comparison of subsp. CECT 219T and KM20 sequenced strains [ PDF] (35 kb)

PDF

(see text of main paper for details): ( subsp. CECT 872 ) [ PDF] (126 kb) ( LC51 ) [ PDF] (241 kb) ( DSM 7378 [ PDF] (411 kb) PCR conditions used to amplify DNA sequences used as probes [ PDF] (24 kb) Physical and genetic map comparison of subsp. CECT 219T and KM20 sequenced strains [ PDF] (35 kb)

PDF

(see text of main paper for details): ( subsp. CECT 872 ) [ PDF] (126 kb) ( LC51 ) [ PDF] (241 kb) ( DSM 7378 [ PDF] (411 kb) PCR conditions used to amplify DNA sequences used as probes [ PDF] (24 kb) Physical and genetic map comparison of subsp. CECT 219T and KM20 sequenced strains [ PDF] (35 kb)

PDF

(see text of main paper for details): ( subsp. CECT 872 ) [ PDF] (126 kb) ( LC51 ) [ PDF] (241 kb) ( DSM 7378 [ PDF] (411 kb) PCR conditions used to amplify DNA sequences used as probes [ PDF] (24 kb) Physical and genetic map comparison of subsp. CECT 219T and KM20 sequenced strains [ PDF] (35 kb)

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