1887

Abstract

The cAMP receptor protein (Crp) is a global transcriptional regulator that binds sequence-specific promoter elements when associated with cAMP. In the motile cyanobacterium sp. strain PCC 6803, intracellular cAMP increases when dark-adapted cells are illuminated. Previous work has established that Crp binds proposed Crp target sites upstream of (), (), (), and , and that is downregulated in a mutant during photoautotrophic growth. To identify additional Crp target genes in , 11 different Crp binding sites proposed during a previous computational survey were tested for sequence-specific binding and -dependent transcription. The results indicate that and can be added as ‘target genes of Sycrp1’ in . Promoter mapping of the targets revealed the same close association of RNA polymerase and Crp as that found in class I and class II Crp-regulated promoters, thereby strongly suggesting similar mechanisms of transcriptional activation.

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2009-09-01
2019-10-14
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