1887

Abstract

is a well-known pathogen in chronic respiratory diseases such as cystic fibrosis. Infectivity of is related to the ability to grow under oxygen-limited conditions using the anaerobic metabolism of denitrification, in which nitrate is reduced to dinitrogen via nitric oxide (NO). Denitrification is activated by a cascade of redox-sensitive transcription factors, among which is the DNR regulator, sensitive to nitrogen oxides. To gain further insight into the mechanism of NO-sensing by DNR, we have developed an -based reporter system to investigate different aspects of DNR activity. In DNR responds to NO, as shown by its ability to transactivate the promoter. The direct binding of DNR to the target DNA is required, since mutations in the helix–turn–helix domain of DNR and specific nucleotide substitutions in the consensus sequence of the promoter abolish the transcriptional activity. Using an strain deficient in haem biosynthesis, we have also confirmed that haem is required for the NO-dependent DNR activity, in agreement with the property of DNR to bind haem . Finally, we have shown, we believe for the first time, that DNR is able to discriminate between different diatomic signal molecules, NO and CO, both ligands of the reduced haem iron , suggesting that DNR responds specifically to NO.

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2009-09-01
2019-10-17
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