1887

Abstract

A-factor (2-isocapryloyl-3-hydroxymethyl--butyrolactone) is a microbial hormone that triggers morphological differentiation and secondary metabolism in . The effects of A-factor on global gene expression were determined by DNA microarray analysis of transcriptomes obtained with the A-factor-deficient mutant Δ. A-factor was added at a concentration of 25 ng ml to mutant Δ at the middle of the exponential growth phase, and RNA samples were prepared from the cells grown after A-factor addition for a further 5, 15 and 30 min, and 1, 2, 4, 8 and 12 h. The effects of A-factor on transcription of all protein-coding genes of were evaluated by comparison of the transcriptomes with those obtained from cells grown in the absence of A-factor. Analysis of variance among the transcriptomes revealed that 477 genes, which were dispersed throughout the chromosome, were differentially expressed during the 12 h after addition of A-factor, when evaluated by specific criteria. Quality threshold clustering analysis with regard to putative polycistronic transcriptional units and levels of upregulation predicted that 152 genes belonging to 74 transcriptional units were probable A-factor-inducible genes. Competitive electrophoretic mobility shift assays using DNA fragments including putative promoter regions of these 74 transcriptional units suggested that AdpA bound 37 regions to activate 72 genes in total. Many of these A-factor-inducible genes encoded proteins of unknown function, suggesting that the A-factor regulatory cascade of affects gene expression at a specific time point more profoundly than expected.

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/content/journal/micro/10.1099/mic.0.027862-0
2009-07-01
2019-12-11
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PCR primers used in this study [PDF file](71 KB)

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Differentially expressed genes 5 min after A-factor addition (expression ratio of either >2.0 or <0.5 and <0.05) [PDF file](25 KB)

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Differentially expressed genes 15 min after A-factor addition (expression ratio of either >2.0 or <0.5 and <0.05) [PDF file](32 KB)

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Differentially expressed genes 30 min after A-factor addition (expression ratio of either >2.0 or <0.5 and <0.05) [PDF file](38 KB)

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Differentially expressed genes 1 h after A-factor addition (expression ratio of either >2.0 or <0.5 and <0.05) [PDF file](43 KB)

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Differentially expressed genes 2 h after A-factor addition (expression ratio of either >2.0 or <0.5 and <0.05) [PDF file](69 KB)

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Differentially expressed genes 4 h after A-factor addition (expression ratio of either >2.0 or <0.5 and <0.05) [PDF file](92 KB)

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Differentially expressed genes 8 h after A-factor addition (expression ratio of either >2.0 or <0.5 and <0.05) [PDF file](143 KB)

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Differentially expressed genes 12 h after A-factor addition (expression ratio of either >2.0 or <0.5 and <0.05) [PDF file](173 KB)

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Genes differentially expressed after addition of A-factor (expression ratio of either >2.0 or <0.5 at least one time point and an ANOVA <0.05) [PDF file](491 KB)

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Genes classified into cluster 1 [PDF file](297 KB)

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Genes classified into cluster 2 [PDF file](84 KB)

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