1887

Abstract

OmpW of serovar Typhimurium has been described as a minor porin involved in osmoregulation, and is also affected by environmental conditions. Biochemical and genetic evidence from our laboratory indicates that OmpW is involved in efflux of and resistance towards paraquat (PQ), and its expression has been shown to be activated in response to oxidative stress. In this study we have explored expression in response to PQ. Primer extension and transcriptional fusions showed that its expression was induced in the presence of PQ. analyses suggested a putative binding site for the SoxS transcriptional factor at the regulatory region. Electrophoretic mobility shift assays (EMSAs) and footprinting experiments showed that SoxS binds at a region that starts close to −54 and ends at about −197 upstream of the transcription start site. Transcriptional fusions support the relevance of this region in activation. The SoxS site is in the forward orientation and its location suggests that the gene has a class I SoxS-dependent promoter.

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2009-08-01
2019-10-20
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