1887

Abstract

Expression of genes involved in the pathogenesis of is controlled by global regulatory loci, including two-component regulatory systems and transcriptional regulators. The staphylococcal-specific SarA family of transcription regulators control large numbers of target genes involved in virulence, autolysis, biofilm formation, stress responses and metabolic processes, and are recognized as potential therapeutic targets. Expression of some of these important regulators has been examined, mostly in laboratory strains, while the pattern of expression of these genes in other strains, especially clinical isolates, is largely unknown. In this report, a comparative analysis of 10 -family genes was conducted in six different strains, including two laboratory (RN6390, SH1000) and four clinical (MW2, Newman, COL and UAMS-1) strains, by Northern and Western blot analyses. Transcription of most of the -family genes showed a strong growth phase-dependence in all strains tested. Among these genes, no difference was observed in expression of the , , and genes, while a major difference was observed in expression of the gene only in strain RN6390. Expression of , , , and was observed in all strains, but the level of expression varied from strain to strain.

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2009-07-01
2019-10-17
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