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Volume 155, Issue 4

Novel subfamily of mitochondrial HMG box-containing proteins: functional analysis of Gcf1p from Free

Abstract

Mitochondria of eukaryotic organisms contain populations of DNA molecules that are packed into higher-order structures called mitochondrial nucleoids (mt-nucleoids). In , the compaction of mitochondrial DNA (mtDNA) into mt-nucleoids is mediated primarily by the high-mobility group (HMG) box-containing protein Abf2, which is an important player in stabilization and metabolism of mtDNA. Although it is evident that analogous proteins must exist in other yeast species, an apparently fast divergence rate has precluded their identification, characterization and comparative analysis. Using analysis of the complete genome sequence of the pathogenic yeast we predicted that the ORF 19.400/19.8030 assigned as encodes a putative mitochondrial HMG box-containing protein. In contrast to Abf2p, which contains two HMG boxes, Gcf1p contains only one C-terminal HMG box. In addition, it contains one putative coiled-coil domain with a potential role in protein dimerization. Fluorescence microscopy analysis of a C-terminally tagged Gcf1p with green fluorescent protein (GFP) revealed its mitochondrial localization in both heterologous () and native () hosts. Biochemical analyses of DNA-binding properties indicate that Gcf1p is, similarly to Abf2p, a non-specific DNA-binding protein. To analyse the role of Gcf1p in mtDNA metabolism, we constructed strains lacking one functional allele of the gene and carrying one allele under the control of the promoter. Under repressible conditions this strain exhibited a more than 3000-fold decrease in levels of mRNA, which was correlated with a substantial decrease in the number of mtDNA copies as well as recombination intermediates. The dramatic effect of reduced levels of Gcf1p on mtDNA metabolism indicates that the protein is involved in essential molecular transactions that relate to the mitochondrial genome.

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Keyword(s): AGE, agarose gel electrophoresis , DAPI, 4′,6-diamidino-2-phenylindole , EMSA, electrophoretic mobility shift assay , GST, glutathione S-transferase , HMG, high-mobility group , mt-nucleoid, mitochondrial nucleoid , mtDNA, mitochondrial DNA , mtHMG, mitochondrial HMG box-containing protein and qRT-PCR, quantitative real-time RT-PCR
SGM
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2009-04-01
2024-04-16
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Novel subfamily of mitochondrial HMG box-containing proteins: functional analysis of Gcf1p from Candida albicans
Microbiology 155, 1226 (2009); https://doi.org/10.1099/mic.0.025759-0
/content/journal/micro/10.1099/mic.0.025759-0
/content/journal/micro/10.1099/mic.0.025759-0
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