1887

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Volume 155, Issue 6

Characterization and subcellular localization of a bacterial flotillin homologue Free

Abstract

The process of endospore formation in is complex, requiring the generation of two distinct cell types, a forespore and larger mother cell. The development of these cell types is controlled and regulated by cell type-specific gene expression, activated by a -factor cascade. Activation of these cell type-specific sigma factors is coupled with the completion of polar septation. Here, we describe a novel protein, YuaG, a eukaryotic reggie/flotillin homologue that is involved in the early stages of sporulation of the Gram-positive model organism . YuaG localizes in discrete foci in the membrane and is highly dynamic. Purification of detergent-resistant membranes revealed that YuaG is associated with negatively charged phospholipids, e.g. phosphatidylglycerol (PG) or cardiolipin (CL). However, localization of YuaG is not always dependent on PG/CL . A disruption strain shows a delay in the onset of sporulation along with reduced sporulation efficiency, where the spores develop to a certain stage and then appear to be trapped at this stage. Our results indicate that YuaG is involved in the early stage of spore development, probably playing a role in the signalling cascade at the onset of sporulation.

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  • Accepted:
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  • Published Online:
Keyword(s): CL, cardiolipin , DRM, detergent-resistant membrane , NAO, 10-N-nonyl-3,6-bis(dimethylamino)acridine orange , PE, phosphatidylethanolamine and PG, phosphatidylglycerol; PI, phosphatidylinositol
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2009-06-01
2024-04-19
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Characterization and subcellular localization of a bacterial flotillin homologue
Microbiology 155, 1786 (2009); https://doi.org/10.1099/mic.0.025312-0
/content/journal/micro/10.1099/mic.0.025312-0
/content/journal/micro/10.1099/mic.0.025312-0
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