%0 Journal Article %A Bosch, Gundula %A Wang, Tiansong %A Latypova, Ekaterina %A Kalyuzhnaya, Marina G. %A Hackett, Murray %A Chistoserdova, Ludmila %T Insights into the physiology of Methylotenera mobilis as revealed by metagenome-based shotgun proteomic analysis %D 2009 %J Microbiology, %V 155 %N 4 %P 1103-1110 %@ 1465-2080 %R https://doi.org/10.1099/mic.0.024968-0 %K GS/GOGAT, glutamine synthetase/glutamate synthase %K RMP, ribulose monophosphate %I Microbiology Society, %X While the shotgun proteomics approach is gaining momentum in understanding microbial physiology, it remains limited by the paucity of high-quality genomic data, especially when it comes to poorly characterized newly identified phyla. At the same time, large-scale metagenomic sequencing projects produce datasets representing genomes of a variety of environmental microbes, although with lower sequence coverage and sequence quality. In this work we tested the utility of a metagenomic dataset enriched in sequences of environmental strains of Methylotenera mobilis, to assess the protein profile of a laboratory-cultivated strain, M. mobilis JLW8, as a proof of principle. We demonstrate that a large portion of the proteome predicted from the metagenomic sequence (approx. 20 %) could be identified with high confidence (three or more peptide sequences), thus gaining insights into the physiology of this bacterium, which represents a new genus within the family Methylophilaceae. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.024968-0