1887

Abstract

In order to gain additional understanding of the physiological mechanisms used by bacteria to maintain surface homeostasis and to identify potential targets for new antibacterial drugs, we analysed the variation of the transcriptional profile in response to inhibitory and subinhibitory concentrations of vancomycin. Our analysis identified 153 genes differentially regulated after exposing bacteria to a concentration of the drug ten times higher than the MIC, and 141 genes differentially expressed when bacteria were growing in a concentration of the drug eightfold lower than the MIC. Hierarchical clustering analysis indicated that the response to these different conditions is different, although with some overlap. This approach allowed us to identify several genes whose products could be involved in the protection from antibiotic stress targeting the envelope and help to confer the basal level of resistance to antibacterial drugs, such as Rv2623 (UspA-like), Rv0116c, PE20-PPE31, PspA and proteins related to toxin–antitoxin systems. Moreover, we also demonstrated that the alternative sigma factor confers basal resistance to vancomycin, once again underlining its importance in the physiology of the mycobacterial surface stress response.

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2009-04-01
2019-09-21
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Genes differentially regulated after exposing bacteria for 1 h to a concentration of vancomycin ten times higher than the MIC [ Excel file] (30 kb) Genes differentially regulated after exposing bacteria for 4 h to a concentration of vancomycin ten times higher than the MIC [ Excel file] (68 kb) Genes differentially regulated when bacteria were growing in a concentration of vancomycin eightfold lower than the MIC [ Excel file] (48 kb) Genes commonly induced in H37Rv under different stress conditions [ PDF] (10 kb) Genes induced in all vancomycin conditions and their induction by other stresses [ PDF] (10 kb)

EXCEL

Genes differentially regulated after exposing bacteria for 1 h to a concentration of vancomycin ten times higher than the MIC [ Excel file] (30 kb) Genes differentially regulated after exposing bacteria for 4 h to a concentration of vancomycin ten times higher than the MIC [ Excel file] (68 kb) Genes differentially regulated when bacteria were growing in a concentration of vancomycin eightfold lower than the MIC [ Excel file] (48 kb) Genes commonly induced in H37Rv under different stress conditions [ PDF] (10 kb) Genes induced in all vancomycin conditions and their induction by other stresses [ PDF] (10 kb)

EXCEL

Genes differentially regulated after exposing bacteria for 1 h to a concentration of vancomycin ten times higher than the MIC [ Excel file] (30 kb) Genes differentially regulated after exposing bacteria for 4 h to a concentration of vancomycin ten times higher than the MIC [ Excel file] (68 kb) Genes differentially regulated when bacteria were growing in a concentration of vancomycin eightfold lower than the MIC [ Excel file] (48 kb) Genes commonly induced in H37Rv under different stress conditions [ PDF] (10 kb) Genes induced in all vancomycin conditions and their induction by other stresses [ PDF] (10 kb)

EXCEL

Genes differentially regulated after exposing bacteria for 1 h to a concentration of vancomycin ten times higher than the MIC [ Excel file] (30 kb) Genes differentially regulated after exposing bacteria for 4 h to a concentration of vancomycin ten times higher than the MIC [ Excel file] (68 kb) Genes differentially regulated when bacteria were growing in a concentration of vancomycin eightfold lower than the MIC [ Excel file] (48 kb) Genes commonly induced in H37Rv under different stress conditions [ PDF] (10 kb) Genes induced in all vancomycin conditions and their induction by other stresses [ PDF] (10 kb)

PDF

Genes differentially regulated after exposing bacteria for 1 h to a concentration of vancomycin ten times higher than the MIC [ Excel file] (30 kb) Genes differentially regulated after exposing bacteria for 4 h to a concentration of vancomycin ten times higher than the MIC [ Excel file] (68 kb) Genes differentially regulated when bacteria were growing in a concentration of vancomycin eightfold lower than the MIC [ Excel file] (48 kb) Genes commonly induced in H37Rv under different stress conditions [ PDF] (10 kb) Genes induced in all vancomycin conditions and their induction by other stresses [ PDF] (10 kb)

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