1887

Abstract

The gene encodes sulphonamide resistance (Sul) and is commonly found in from different hosts. We typed isolates by multilocus sequence typing (MLST) and compared the results to sequence variation of , in order to investigate the relation to host origin of pathogenic and commensal strains and to investigate whether transfer of into different genomic lineages has happened multiple times. Sixty-eight isolated in Denmark and Norway from different hosts and years were MLST typed and PCR products were sequenced and compared. PFGE was performed in a subset of isolates. All isolates were divided into 45 different sequence types (STs), with clonal complexes CC10, CC23, CC168, CC350 and CC69 being the most frequent. The gene from the majority of strains had only two point mutations, at positions 159 and 197, leading to a synonymous and a non-synonymous change, respectively. Five strains had extra single mutations. All poultry, poultry meat, and Danish human blood isolates had the same ST and some of these strains clustered under the same MLST STs, indicating that they shared habitats. Most PFGE profiles clustered according to source, but some included different sources. Sul from different animals, food, human faeces and infections did not cluster according to their origin, suggesting that these habitats share and gene types. However, while pig isolates on one occasion clustered with urinary tract infection isolates, poultry isolates seemed more related to isolates from bloodstream infections in humans. Presence of mainly two types of the gene in both human and animal isolates, irrespective of date and geography, and the presence of both types in the same clonal lineages, suggest horizontal transfer of .

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.024190-0
2009-03-01
2020-01-21
Loading full text...

Full text loading...

/deliver/fulltext/micro/155/3/831.html?itemId=/content/journal/micro/10.1099/mic.0.024190-0&mimeType=html&fmt=ahah

References

  1. Blahna, M. T., Zalewski, C. A., Reuer, J., Kahlmeter, G., Foxman, B. & Marrs, C. F. ( 2006; ). The role of horizontal gene transfer in the spread of trimethoprim-sulfamethoxazole resistance among uropathogenic Escherichia coli in Europe and Canada. J Antimicrob Chemother 57, 666–672.[CrossRef]
    [Google Scholar]
  2. Feil, E. J., Li, B. C., Aanensen, D. M., Hanage, W. P. & Spratt, B. G. ( 2004; ). eBURST: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data. J Bacteriol 186, 1518–1530.[CrossRef]
    [Google Scholar]
  3. Gow, S. P., Waldner, C. L., Harel, J. & Boerlin, P. ( 2008; ). Associations between antimicrobial resistance genes in fecal generic Escherichia coli isolates in western Canadian cow-calf herds. Appl Environ Microbiol 74, 3658–3666.[CrossRef]
    [Google Scholar]
  4. Grape, M., Sundstrom, L. & Kronvall, G. ( 2003; ). Sulphonamide resistance gene sul3 found in Escherichia coli isolates from human sources. J Antimicrob Chemother 52, 1022–1024.[CrossRef]
    [Google Scholar]
  5. Hammerum, A. M., Sandvang, D., Andersen, S. R., Seyfarth, A. M., Porsbo, L. J., Frimodt-Moller, N. & Heuer, O. E. ( 2006; ). Detection of sul1, sul2 and sul3 in sulphonamide resistant Escherichia coli isolates obtained from healthy humans, pork and pigs in Denmark. Int J Food Microbiol 106, 235–237.[CrossRef]
    [Google Scholar]
  6. Kerrn, M. B., Klemmensen, T., Frimodt-Moller, N. & Espersen, F. ( 2002; ). Susceptibility of Danish Escherichia coli strains isolated from urinary tract infections and bacteraemia, and distribution of sul genes conferring sulphonamide resistance. J Antimicrob Chemother 50, 513–516.[CrossRef]
    [Google Scholar]
  7. Radstrom, P. & Swedberg, G. ( 1988; ). RSF1010 and a conjugative plasmid contain sulII, one of two known genes for plasmid-borne sulfonamide resistance dihydropteroate synthase. Antimicrob Agents Chemother 32, 1684–1692.[CrossRef]
    [Google Scholar]
  8. Sorum, H. & L'Abee-Lund, T. M. ( 2002; ). Antibiotic resistance in food-related bacteria – a result of interfering with the global web of bacterial genetics. Int J Food Microbiol 78, 43–56.[CrossRef]
    [Google Scholar]
  9. Sunde, M. & Norstrom, M. ( 2006; ). The prevalence of, associations between and conjugal transfer of antibiotic resistance genes in Escherichia coli isolated from Norwegian meat and meat products. J Antimicrob Chemother 58, 741–747.[CrossRef]
    [Google Scholar]
  10. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  11. Tartof, S. Y., Solberg, O. D., Manges, A. R. & Riley, L. W. ( 2005; ). Analysis of a uropathogenic Escherichia coli clonal group by multilocus sequence typing. J Clin Microbiol 43, 5860–5864.[CrossRef]
    [Google Scholar]
  12. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x-Windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  13. Trobos, M., Jakobsen, L., Olsen, K. E., Frimodt-Moller, N., Hammerum, A. M., Pedersen, K., Agerso, Y., Porsbo, L. J. & Olsen, J. E. ( 2008; ). Prevalence of sulphonamide resistance and class 1 integron genes in Escherichia coli isolates obtained from broilers, broiler meat, healthy humans and urinary infections in Denmark. Int J Antimicrob Agents 32, 367–369.[CrossRef]
    [Google Scholar]
  14. Turner, K. M. & Feil, E. J. ( 2007; ). The secret life of the multilocus sequence type. Int J Antimicrob Agents 29, 129–135.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.024190-0
Loading
/content/journal/micro/10.1099/mic.0.024190-0
Loading

Data & Media loading...

Supplements

UPGMA dendrogram of I PFGE patterns of isolates from poultry, poultry meat and human blood infections [ PDF] (302 kb) Summary dataset of all the 68 isolates included in this study [ PDF] (22 kb) MLST sequence types (ST) and clonal complexes (CC) of strains containing the gene found across species [ PDF] (13 kb)

PDF

UPGMA dendrogram of I PFGE patterns of isolates from poultry, poultry meat and human blood infections [ PDF] (302 kb) Summary dataset of all the 68 isolates included in this study [ PDF] (22 kb) MLST sequence types (ST) and clonal complexes (CC) of strains containing the gene found across species [ PDF] (13 kb)

PDF

UPGMA dendrogram of I PFGE patterns of isolates from poultry, poultry meat and human blood infections [ PDF] (302 kb) Summary dataset of all the 68 isolates included in this study [ PDF] (22 kb) MLST sequence types (ST) and clonal complexes (CC) of strains containing the gene found across species [ PDF] (13 kb)

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error