1887

Abstract

Systematic screening of secretion proteins using an approach based on the completely sequenced genome of R revealed 405 candidate signal peptides, 108 of which were able to heterologously secrete an active-form -amylase derived from . These comprised 90 general secretory (Sec)-type, 10 twin-arginine translocator (Tat)-type and eight Sec-type with presumptive lipobox peptides. Only Sec- and Tat-type signals directed high-efficiency secretion. In two assays, 11 of these signals resulted in 50- to 150-fold increased amounts of secreted -amylase compared with the well-known corynebacterial secretory protein PS2. While the presence of an AXA motif at the cleavage sites was readily apparent, it was the presence of a glutamine residue adjacent to the cleavage site that may affect secretion efficiency.

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2009-03-01
2019-10-22
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