1887

Abstract

In , the gene encodes a small non-translated RNA that regulates several genes involved in transport of amino acids and peptides (including , and ). Microarray analysis identified as an additional regulatory target of GcvB. The gene encodes a permease for the transport of glycine, -alanine, -serine and -cycloserine. RT-PCR confirmed that GcvB and the Hfq protein negatively regulate mRNA in cells grown in Luria–Bertani broth. In addition, deletion of the gene resulted in increased sensitivity to -cycloserine, consistent with increased expression of . A  : :  translational fusion confirmed that GcvB negatively regulates expression in Luria–Bertani broth and that Hfq is required for the GcvB effect. GcvB had no effect on  : :  expression in glucose minimal medium supplemented with glycine. However, Hfq still negatively regulated the fusion in the absence of GcvB. A set of transcriptional fusions of to identified a sequence in necessary for regulation by GcvB. Analysis of GcvB identified a region complementary to this region of mRNA. However, mutations predicted to disrupt base-pairing between mRNA and GcvB did not alter expression of  : : . A model for GcvB function in cell physiology is discussed.

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2009-01-01
2021-04-15
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