Conjugated linoleic acid (CLA) is formed from linoleic acid (LA; cis-9,cis-12-18 : 2) by intestinal bacteria. Different CLA isomers have different implications for human health. The aim of this study was to investigate LA metabolism and the CLA isomers formed in two individuals (V1 and V2) with different faecal metabolic characteristics, and to compare fatty acid metabolism with the microbial community composition. LA incubated with faecal samples was metabolized at similar rates with both subjects, but the products were different. LA was metabolized extensively to stearic acid (SA; 18 : 0) in V1, with minor accumulation of CLA and more rapid accumulation of vaccenic acid (VA; trans-11-18 : 1). CLA accumulation at 4 h was almost tenfold higher with V2, and little SA was formed. At least 12 different isomers of CLA were produced from LA by the colonic bacteria from the two individuals. The predominant (>75 %) CLA isomer in V1 was rumenic acid (RA; cis-9,trans-11-18 : 2), whereas the concentrations of RA and trans-10,cis-12-18 : 2 were similar with V2. Propionate and butyrate proportions in short-chain fatty acids were higher in V1. A 16S rRNA clone library from V1 contained mainly Bacteroidetes (54 % of clones), whereas Firmicutes (66 % of clones) predominated in V2. Both samples were devoid of bacteria related to Clostridium proteoclasticum, the only gut bacterium known to metabolize VA to SA. Thus, the CLA formed in the intestine of different individuals may differ according to their resident microbiota, with possibly important implications with respect to gut health.
AlonsoL.,
CuestaE. P.,
GillilandS. E.2003; Production of free conjugated linoleic acid by Lactobacillus acidophilus and Lactobacillus casei of human intestinal origin. J Dairy Sci 86:1941–1946
BarcenillaA.,
PrydeS. E.,
MartinJ. C.,
DuncanS. H.,
StewartC. S.,
HendersonC.,
FlintH. J.2000; Phylogenetic relationships of butyrate-producing bacteria from the human gut. Appl Environ Microbiol 66:1654–1661
Bassaganya-RieraJ.,
HontecillasR.2006; CLA and n-3 PUFA differentially modulate clinical activity and colonic PPAR-responsive gene expression in a pig model of experimental IBD. Clin Nutr 25:454–465
BeppuF.,
HosokawaM.,
TanakaL.,
KohnoH.,
TanakaT.,
MiyashitaK.2006; Potent inhibitory effect of trans -9, trans -11 isomer of conjugated linoleic acid on the growth of human colon cancer cells. J Nutr Biochem 17:830–836
ChinS. F.,
StorksonJ. M.,
LiuW.,
AlbrightK. J.,
ParizaM. W.1994; Conjugated linoleic acid (9,11- and 10,12-octodecadienoic acid) is produced in conventional but not germ-free rats fed linoleic acid. J Nutr 124:694–701
ChoH. J.,
KimW. K.,
JungJ. I.,
KimE. J.,
LimS. S.,
KwonD. Y.,
ParkJ. H.2005; trans -10, cis -12, not cis -9, trans -11, conjugated linoleic acid decreases ErbB3 expression in HT-29 human colon cancer cells. World J Gastroenterol 11:5142–5150
ColeJ. R.,
ChaiB.,
FarrisR. J.,
WangQ.,
KulamS. A.,
McGarrellD. M.,
GarrityG. M.,
TiedjeJ. M.2005; The Ribosomal Database Project (RDP-II): sequences and tools for high-throughput rRNA analysis. Nucleic Acids Res 33:D294–D296
DevillardE.,
McIntoshF. M.,
NewboldC. J.,
WallaceR. J.2006; Rumen ciliate protozoa contain high concentrations of conjugated linoleic acids and vaccenic acid, yet do not hydrogenate linoleic acid or desaturate stearic acid. Br J Nutr 96:697–704
DevillardE.,
McIntoshF. M.,
DuncanS. H.,
WallaceR. J.2007; Metabolism of linoleic acid by human gut bacteria: different routes for biosynthesis of conjugated linoleic acid. J Bacteriol 189:2566–2570
EckburgP. B.,
BikE. M.,
BernsteinC. N.,
PurdomE.,
DethlefsenL.,
SargentM.,
GillS. R.,
NelsonK. E.,
RelmanD. A.2005; Diversity of the human intestinal microbial flora. Science 308:1635–1638
EwaschukJ. B.,
WalkerJ. W.,
DiazH.,
MadsenK. L.2006; Bioproduction of conjugated linoleic acid by probiotic bacteria occurs in vitro and in vivo in mice. J Nutr 136:1483–1487
FayL.,
RichliU.1991; Location of double bonds in polyunsaturated fatty acids by gas chromatography-mass spectrometry after 4,4-dimethyloxazoline derivatization. J Chromatog 541:89–98
FlintH. J.,
DuncanS. H.,
ScottK. P.,
LouisP.2007; Interactions and competition within the microbial community of the human colon: links between diet and health. Environ Microbiol 9:1101–1111
HarfootC. G.,
HazlewoodG. P.1997; Lipid metabolism in the rumen. In The Rumen Microbial Ecosystem pp 348–426 Edited by
HobsonP. N.,
StewartC. S.
London: Chapman & Hall;
HauptmanJ.,
LucasC.,
BoldrinM. N.,
CollinsH.,
SegalK. R.2000; Orlistat in the long-term treatment of obesity in primary care settings. Arch Fam Med 9:160–167
HayashiH.,
TakahashiR.,
NishiT.,
SakamotoM.,
BennoY.2005; Molecular analysis of jejunal, ileal, caecal and recto-sigmoidal human colonic microbiota using 16S rRNA gene libraries and terminal restriction fragment length polymorphism. J Med Microbiol 54:1093–1101
KamlageB.,
HartmannL.,
GruhlB.,
BlautM.2000; Linoleic acid conjugation by human intestinal microorganisms is inhibited by glucose and other substrates in vitro and in gnotobiotic rats. J Nutr 130:2036–2039
LampenA.,
LeifheitM.,
VossJ.,
NauH.2005; Molecular and cellular effects of cis -9, trans -11-conjugated linoleic acid in enterocytes: effects on proliferation, differentiation, and gene expression. Biochim Biophys Acta173530–40
LawsonR. E.,
MossA. R.,
GivensD. I.2001; The role of dairy products in supplying conjugated linoleic acid to man's diet: a review. Nutr Res Rev 14:153–172
MarteauP.,
PochartP.,
DoreJ.,
Bera-MailletC.,
BernalierA.,
CorthierG.2001; Comparative study of bacterial groups within the human cecal and fecal microbiota. Appl Environ Microbiol 67:4939–4942
RichardsonA. J.,
CalderG. C.,
StewartC. S.,
SmithA.1989; Simultaneous determination of volatile and non-volatile fermentation products of anaerobes by capillary gas chromatography. Lett Appl Microbiol 9:5–8
ShingfieldK. J.,
AhvenjärviS.,
ToivonenV.,
ÄröläA.,
NurmelaK. V. V.,
HuhtanenP.,
GriinariJ. M.2003; Effect of dietary fish oil on biohydrogenation of fatty acids and milk fatty acid content in cows. Anim Sci 77:165–180
ShingfieldK. J.,
ReynoldsC. K.,
HervásG.,
GriinariJ. M.,
GrandisonA. S.,
BeeverD. E.2006; Examination of the persistency of milk fatty acid composition responses to fish oil and sunflower oil in the diet of dairy cows. J Dairy Sci 89:714–732
SuauA.,
BonnetR.,
SutrenM.,
GodonJ. J.,
GibsonG. R.,
CollinsM. D.,
DoreJ.1999; Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Appl Environ Microbiol 65:4799–4807
TriconS.,
BurdgeG. C.,
WilliamsC. M.,
CalderP. C.,
YaqoobP.2005; The effects of conjugated linoleic acid on human health-related outcomes. Proc Nutr Soc 64:171–182
WallaceR. J.,
ChaudharyL. C.,
McKainN.,
McEwanN. R.,
RichardsonA. J.,
VercoeP. E.,
WalkerN. D.,
PaillardD.2006; Clostridium proteoclasticum : a ruminal bacterium that forms stearic acid from linoleic acid. FEMS Microbiol Lett 265:195–201
WaşowskaI.,
MaiaM. R. G.,
NiedzwiedzkaK. M.,
CzaudernaM.,
Ramalho RibeiroJ. M. C.,
DevillardE.,
ShingfieldK. J.,
WallaceR. J.2006; Influence of fish oil on ruminal biohydrogenation of C18 unsaturated fatty acids. Br J Nutr 95:1199–1211
ZoetendalE. G.,
AkkermansA. D.,
De VosW. M.1998; Temperature gradient gel electrophoresis analysis of 16S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria. Appl Environ Microbiol 64:3854–3859