RT Journal Article SR Electronic(1) A1 Zhang, Yongshu A1 Whiteley, Marvin A1 Kreth, Jens A1 Lei, Yu A1 Khammanivong, Ali A1 Evavold, Jamie N. A1 Fan, Jingyuan A1 Herzberg, Mark C.YR 2009 T1 The two-component system BfrAB regulates expression of ABC transporters in Streptococcus gordonii and Streptococcus sanguinis JF Microbiology, VO 155 IS 1 SP 165 OP 173 DO https://doi.org/10.1099/mic.0.023168-0 PB Microbiology Society, SN 1465-2080, AB The putative two-component system BfrAB is involved in Streptococcus gordonii biofilm development. Here, we provide evidence that BfrAB regulates the expression of bfrCD and bfrEFG, which encode two ATP-binding cassette (ABC) transporters, and bfrH, which encodes a CAAX amino-terminal protease family protein. BfrC and BfrE are ATP-binding proteins, and BfrD, BfrF and BfrG are homologous membrane-spanning polypeptides. Similarly, BfrABss, the BfrAB homologous system in Streptococcus sanguinis, controls the expression of two bfrCD-homologous operons (bfrCD ss and bfrXY ss), a bfrH-homologous gene (bfrH1 ss) and another CAAX amino-terminal protease family protein gene (bfrH2ss ). Furthermore, we demonstrate that the purified BfrA DNA-binding domain from S. gordonii binds to the promoter regions of bfrCD, bfrEFG, bfrH, bfrCD ss, bfrXY ss and bfrH1 ss in vitro. Finally, we show that the BfrA DNA-binding domain recognizes a conserved DNA motif with a consensus sequence of TTTCTTTAGAAATATTTTAGAATT. These data suggest, therefore, that S. gordonii BfrAB controls biofilm formation by regulating multiple ABC-transporter systems., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.023168-0