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Microbiology Society logo Microbiology

Volume 155, Issue 1

The two-component system BfrAB regulates expression of ABC transporters in and Free

Abstract

The putative two-component system BfrAB is involved in biofilm development. Here, we provide evidence that BfrAB regulates the expression of and , which encode two ATP-binding cassette (ABC) transporters, and , which encodes a CAAX amino-terminal protease family protein. BfrC and BfrE are ATP-binding proteins, and BfrD, BfrF and BfrG are homologous membrane-spanning polypeptides. Similarly, BfrAB, the BfrAB homologous system in , controls the expression of two -homologous operons ( and ), a -homologous gene ( ) and another CAAX amino-terminal protease family protein gene (). Furthermore, we demonstrate that the purified BfrA DNA-binding domain from binds to the promoter regions of , , , , and . Finally, we show that the BfrA DNA-binding domain recognizes a conserved DNA motif with a consensus sequence of TTTCTTTAGAAATATTTTAGAATT. These data suggest, therefore, that BfrAB controls biofilm formation by regulating multiple ABC-transporter systems.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): ABC, ATP-binding cassette , EMSA, electrophoretic mobility shift assay and TCS, two-component system
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2009-01-01
2025-04-25
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/content/journal/micro/10.1099/mic.0.023168-0
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The two-component system BfrAB regulates expression of ABC transporters in Streptococcus gordonii and Streptococcus sanguinis
Microbiology 155, 165 (2009); https://doi.org/10.1099/mic.0.023168-0
/content/journal/micro/10.1099/mic.0.023168-0
/content/journal/micro/10.1099/mic.0.023168-0
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