1887

Abstract

The putative two-component system BfrAB is involved in biofilm development. Here, we provide evidence that BfrAB regulates the expression of and , which encode two ATP-binding cassette (ABC) transporters, and , which encodes a CAAX amino-terminal protease family protein. BfrC and BfrE are ATP-binding proteins, and BfrD, BfrF and BfrG are homologous membrane-spanning polypeptides. Similarly, BfrAB, the BfrAB homologous system in , controls the expression of two -homologous operons ( and ), a -homologous gene ( ) and another CAAX amino-terminal protease family protein gene (). Furthermore, we demonstrate that the purified BfrA DNA-binding domain from binds to the promoter regions of , , , , and . Finally, we show that the BfrA DNA-binding domain recognizes a conserved DNA motif with a consensus sequence of TTTCTTTAGAAATATTTTAGAATT. These data suggest, therefore, that BfrAB controls biofilm formation by regulating multiple ABC-transporter systems.

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2009-01-01
2019-10-17
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