1887

Abstract

Colibacillosis is a common systemic disease of worldwide economic importance in poultry, caused by . are normally found in the intestines of poultry, but some strains are able to cause extraintestinal disease. Plasmid pVM01 is essential for virulence in avian pathogenic (APEC) strain E3 in chickens after aerosol exposure and contains the virulence-associated genes , and in distinct regions. The determination of the complete sequence of this plasmid identified many ORFs that were highly similar to genes found in the APEC O1 plasmid, as well as many hypothetical ORFs. Truncated versions of pVM01 were constructed and introduced into avirulent APEC strain E3/2.4 and the pathogenicity of these strains was assessed by aerosol exposure. The function of the region of pVM01 that contains the genes for conjugation was confirmed. Strains carrying the truncated plasmids appeared to be of intermediate virulence compared to the wild-type APEC strain E3. The conserved portion of the putative virulence region was found to contribute to the colonization of and generation of lesions in the air sacs. Both the conserved and variable portions of the putative virulence region were shown to contribute to the colonization of the trachea, but the variable portion of the putative virulence region was not required for the strain to confer a virulent phenotype. These results reveal that deletion of the conserved portion of the putative virulence region, but not the variable portion of the putative virulence region, is associated with a decrease in virulence of APEC.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.023143-0
2009-02-01
2019-12-12
Loading full text...

Full text loading...

/deliver/fulltext/micro/155/2/450.html?itemId=/content/journal/micro/10.1099/mic.0.023143-0&mimeType=html&fmt=ahah

References

  1. Barnes, H. J., Nolan, L. K. & Vaillancourt, J.-P. ( 2008; ). Colibacillosis. In Diseases of Poultry, pp. 691–737. Edited by Y. M. Saif: Blackwell Publishing.
  2. Bell, S. M. ( 1984; ). Antibiotic sensitivity testing by the CDS method. Clinical Microbiology Update Program – 1984. Edited by N. Hartwig. Sydney, Australia: NSW Branch of the Australian Society for Microbiology.
  3. Chouikha, I., Germon, P., Bree, A., Gilot, P., Moulin-Schouleur, M. & Schouler, C. ( 2006; ). A selC-associated genomic island of the extraintestinal avian pathogenic Escherichia coli strain BEN2908 is involved in carbohydrate uptake and virulence. J Bacteriol 188, 977–987.[CrossRef]
    [Google Scholar]
  4. de Lorenzo, V., Bindereif, A., Paw, B. H. & Neilands, J. B. ( 1986; ). Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12. J Bacteriol 165, 570–578.
    [Google Scholar]
  5. Doetkott, D. M., Nolan, L. K., Giddings, C. W. & Berryhill, D. L. ( 1996; ). Large plasmids of avian Escherichia coli isolates. Avian Dis 40, 927–930.[CrossRef]
    [Google Scholar]
  6. Dozois, C. M., Daigle, F. & Curtiss, R., III ( 2003; ). Identification of pathogen-specific and conserved genes expressed in vivo by an avian pathogenic Escherichia coli strain. Proc Natl Acad Sci U S A 100, 247–252.[CrossRef]
    [Google Scholar]
  7. Germon, P., Chen, Y. H., He, L., Blanco, J. E., Bree, A., Schouler, C., Huang, S. H. & Moulin-Schouleur, M. ( 2005; ). ibeA, a virulence factor of avian pathogenic Escherichia coli. Microbiology 151, 1179–1186.[CrossRef]
    [Google Scholar]
  8. Gibbs, P. S., Petermann, S. R. & Wooley, R. E. ( 2004; ). Comparison of several challenge models for studies in avian colibacillosis. Avian Dis 48, 751–758.[CrossRef]
    [Google Scholar]
  9. Gilson, L., Mahanty, H. K. & Kolter, R. ( 1987; ). Four plasmid genes are required for colicin V synthesis, export and immunity. J Bacteriol 169, 2466–2470.
    [Google Scholar]
  10. Ginns, C. A., Browning, G., Benham, M. & Whithear, K. ( 1998; ). Development and application of an aerosol challenge method for reproduction of avian colibacillosis. Avian Pathol 27, 505–511.[CrossRef]
    [Google Scholar]
  11. Ginns, C. A., Benham, M. L., Adams, L. M., Whithear, K. G., Bettelheim, K. A., Crabb, B. S. & Browning, G. F. ( 2000; ). Colonization of the respiratory tract by a virulent strain of avian Escherichia coli requires carriage of a conjugative plasmid. Infect Immun 68, 1535–1541.[CrossRef]
    [Google Scholar]
  12. Hacker, J., Blum-Oehler, G., Muhldorfer, I. & Tschape, H. ( 1997; ). Pathogenicity islands of virulent bacteria: structure, function and impact on microbial evolution. Mol Microbiol 23, 1089–1097.[CrossRef]
    [Google Scholar]
  13. Hantke, K., Nicholson, G., Rabsch, W. & Winkelmann, G. ( 2003; ). Salmochelins, siderophores of Salmonella enterica and uropathogenic Escherichia coli strains, are recognized by the outer membrane receptor IroN. Proc Natl Acad Sci U S A 100, 3677–3682.[CrossRef]
    [Google Scholar]
  14. Horne, S. M., Pfaff-McDonough, S. J., Giddings, C. W. & Nolan, L. K. ( 2000; ). Cloning and sequencing of the iss gene from a virulent avian Escherichia coli. Avian Dis 44, 179–184.[CrossRef]
    [Google Scholar]
  15. Ideses, D., Gophna, U., Paitan, Y., Chaudhuri, R. R., Pallen, M. J. & Ron, E. Z. ( 2005; ). A degenerate type III secretion system from septicemic Escherichia coli contributes to pathogenesis. J Bacteriol 187, 8164–8171.[CrossRef]
    [Google Scholar]
  16. Ike, K., Kawahara, K., Danbara, H. & Kume, K. ( 1992; ). Serum resistance and aerobactin iron uptake in avian Escherichia coli mediated by conjugative 100-megadalton plasmid. J Vet Med Sci 54, 1091–1098.[CrossRef]
    [Google Scholar]
  17. Johnson, T. J., Johnson, S. J. & Nolan, L. K. ( 2006a; ). Complete DNA sequence of a ColBM plasmid from avian pathogenic Escherichia coli suggests that it evolved from closely related ColV virulence plasmids. J Bacteriol 188, 5975–5983.[CrossRef]
    [Google Scholar]
  18. Johnson, T. J., Siek, K. E., Johnson, S. J. & Nolan, L. K. ( 2006b; ). DNA sequence of a ColV plasmid and prevalence of selected plasmid-encoded virulence genes among avian Escherichia coli strains. J Bacteriol 188, 745–758.[CrossRef]
    [Google Scholar]
  19. Kariyawasam, S., Johnson, T. J. & Nolan, L. K. ( 2006; ). Unique DNA sequences of avian pathogenic Escherichia coli isolates as determined by genomic suppression subtractive hybridization. FEMS Microbiol Lett 262, 193–200.[CrossRef]
    [Google Scholar]
  20. La Ragione, R. M. & Woodward, M. J. ( 2002; ). Virulence factors of Escherichia coli serotypes associated with avian colisepticaemia. Res Vet Sci 73, 27–35.[CrossRef]
    [Google Scholar]
  21. Li, G., Laturnus, C., Ewers, C. & Wieler, L. H. ( 2005; ). Identification of genes required for avian Escherichia coli septicemia by signature-tagged mutagenesis. Infect Immun 73, 2818–2827.[CrossRef]
    [Google Scholar]
  22. Lukashin, A. V. & Borodovsky, M. ( 1998; ). GeneMark.hmm: new solutions for gene finding. Nucleic Acids Res 26, 1107–1115.[CrossRef]
    [Google Scholar]
  23. Mori, H., Mori, Y., Ichinose, C., Niki, H., Ogura, T., Kato, A. & Hiraga, S. ( 1989; ). Purification and characterization of SopA and SopB proteins essential for F plasmid partitioning. J Biol Chem 264, 15535–15541.
    [Google Scholar]
  24. Parreira, V. R. & Gyles, C. L. ( 2003; ). A novel pathogenicity island integrated adjacent to the thrW tRNA gene of avian pathogenic Escherichia coli encodes a vacuolating autotransporter toxin. Infect Immun 71, 5087–5096.[CrossRef]
    [Google Scholar]
  25. Provence, D. L. & Curtiss, R., III ( 1994; ). Isolation and characterization of a gene involved in hemagglutination by an avian pathogenic Escherichia coli strain. Infect Immun 62, 1369–1380.
    [Google Scholar]
  26. Sabri, M., Leveille, S. & Dozois, C. M. ( 2006; ). A SitABCD homologue from an avian pathogenic Escherichia coli strain mediates transport of iron and manganese and resistance to hydrogen peroxide. Microbiology 152, 745–758.[CrossRef]
    [Google Scholar]
  27. Sabri, M., Caza, M., Proulx, J., Lymberopoulos, M. H., Bree, A., Moulin-Schouleur, M., Curtiss, R., III & Dozois, C. M. ( 2008; ). Contribution of the SitABCD, MntH, and FeoB metal transporters to the virulence of avian pathogenic Escherichia coli O78 strain χ7122. Infect Immun 76, 601–611.[CrossRef]
    [Google Scholar]
  28. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbour, NY: Cold Spring Harbor Laboratory.
  29. Schmidt, H. & Hensel, M. ( 2004; ). Pathogenicity islands in bacterial pathogenesis. Clin Microbiol Rev 17, 14–56.[CrossRef]
    [Google Scholar]
  30. Schouler, C., Koffmann, F., Amory, C., Leroy-Setrin, S. & Moulin-Schouleur, M. ( 2004; ). Genomic subtraction for the identification of putative new virulence factors of an avian pathogenic Escherichia coli strain of O2 serogroup. Microbiology 150, 2973–2984.[CrossRef]
    [Google Scholar]
  31. Skyberg, J. A., Johnson, T. J., Johnson, J. R., Clabots, C., Logue, C. M. & Nolan, L. K. ( 2006; ). Acquisition of avian pathogenic Escherichia coli plasmids by a commensal E. coli isolate enhances its abilities to kill chicken embryos, grow in human urine and colonize the murine kidney. Infect Immun 74, 6287–6292.[CrossRef]
    [Google Scholar]
  32. Skyberg, J. A., Johnson, T. J. & Nolan, L. K. ( 2008; ). Mutational and transcriptional analyses of an avian pathogenic Escherichia coli ColV plasmid. BMC Microbiol 8, 24 [CrossRef]
    [Google Scholar]
  33. Tatusova, T. A. & Madden, T. L. ( 1999; ). blast 2 Sequences, a new tool for comparing protein and nucleotide sequences. FEMS Microbiol Lett 174, 247–250.[CrossRef]
    [Google Scholar]
  34. Tivendale, K. A., Allen, J. L., Ginns, C. A., Crabb, B. S. & Browning, G. F. ( 2004; ). Association of iss and iucA, but not tsh, with plasmid-mediated virulence of avian pathogenic Escherichia coli. Infect Immun 72, 6554–6560.[CrossRef]
    [Google Scholar]
  35. Zhou, D., Hardt, W. D. & Galan, J. E. ( 1999; ). Salmonella typhimurium encodes a putative iron transport system within the centisome 63 pathogenicity island. Infect Immun 67, 1974–1981.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.023143-0
Loading
/content/journal/micro/10.1099/mic.0.023143-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error