1887

Abstract

Cyclopropane fatty acyl synthases (CFA synthases) are enzymes that catalyse the addition of a methylene group across double bonds of monounsaturated fatty acyl chains in lipids. We have investigated the function of two putative genes, and proposed to code for CFA synthases in . Total fatty acid composition and fatty acid distributions within lipid classes for wild-type and and mutant strains grown under P starvation and in acidic culture conditions were obtained by GC/MS and by infusion ESI/MS/MS, respectively. For wild-type cells and the mutant, total cyclopropane fatty acids (CFAs) increased by 10 % and 15 % under P starvation and acidic conditions, respectively; whereas in the mutant, CFAs were less than 0.1 % of wild-type under both growth conditions. Reporter gene fusion experiments revealed that and were expressed at similar levels in free-living cells. Thus under the conditions we examined, was required for the cyclopropanation of lipids in whereas the role of remains to be determined. Analysis of intact lipids revealed that cyclopropanation occurred on -11-octadecenoic acid located in either the -1 or the -2 position in phospholipids and that cyclopropanation in the -2 position occurred to a greater extent in phosphatidylcholines and sulfoquinovosyldiacylglycerols under acidic conditions than under P starvation. The gene was also required for cyclopropanation of non-phosphorus-containing lipids. Principal components analysis revealed no differences in the cyclopropanation of four lipid classes. We concluded that cyclopropanation occurred independently of the polar head group. Neither nor was required for symbiotic nitrogen fixation.

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2009-02-01
2020-01-21
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Fatty acid compositions obtained by GC/MS analyses for three bacterial cultures grown over a period of 1 year [ PDF] (18 kb) Alignment of CFA synthase orthologues from (Cfa1 and Cfa2), (AAC74733), [CAA15777 (Cmaa1), CAB00929 (Cmaa2)] and [PP2734 (CfaA) and PP5365 (CfaB)] [ PDF] (302 kb)

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Fatty acid compositions obtained by GC/MS analyses for three bacterial cultures grown over a period of 1 year [ PDF] (18 kb) Alignment of CFA synthase orthologues from (Cfa1 and Cfa2), (AAC74733), [CAA15777 (Cmaa1), CAB00929 (Cmaa2)] and [PP2734 (CfaA) and PP5365 (CfaB)] [ PDF] (302 kb)

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