1887

Abstract

is a foodborne pathogen able to infect humans and many other mammalian species, leading to serious, often fatal disease. We have previously identified a five-gene locus in the genome of EGD-e which comprised three contiguous genes encoding paralogous type I signal peptidases. In the present study, we focused on the two distal genes of the locus ( and ), encoding proteins sharing significant similarities with the YlqF and RnhB proteins, respectively, of . could complement an thermosensitive growth phenotype, suggesting that it encodes a functional RNase H. Strikingly, inactivation of provoked a strong attenuation of virulence in the mouse model, and kinetic studies in infected mice revealed that multiplication of the mutant in target organs was significantly impaired. However, the mutation did not impair intracellular multiplication or cell-to-cell spread in cell culture models. Transcriptional profiles obtained with an -overexpressing strain were compared to those of the wild-type strain, using microarray analyses. The data obtained suggest a pleiotropic regulatory role of Lmo1273 and possible links with amino acid uptake.

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2009-03-01
2019-10-18
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Primers used in this study [ PDF] (51 kb) Genetic organization of the region in other species [ PDF] (49 kb) Growth of in BHI [ PDF] (114 kb) Correlation of microarray and real-time RT-PCR analyses of selected genes [ PDF] (13 kb)

PDF

Primers used in this study [ PDF] (51 kb) Genetic organization of the region in other species [ PDF] (49 kb) Growth of in BHI [ PDF] (114 kb) Correlation of microarray and real-time RT-PCR analyses of selected genes [ PDF] (13 kb)

PDF

Primers used in this study [ PDF] (51 kb) Genetic organization of the region in other species [ PDF] (49 kb) Growth of in BHI [ PDF] (114 kb) Correlation of microarray and real-time RT-PCR analyses of selected genes [ PDF] (13 kb)

PDF

Primers used in this study [ PDF] (51 kb) Genetic organization of the region in other species [ PDF] (49 kb) Growth of in BHI [ PDF] (114 kb) Correlation of microarray and real-time RT-PCR analyses of selected genes [ PDF] (13 kb)

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