1887

Abstract

A Ca signal is required for the process of heterocyst differentiation in the filamentous diazotrophic cyanobacterium sp. PCC 7120. This paper presents evidence that a transient increase in intracellular free Ca is also involved in acclimation to nitrogen starvation in the unicellular non-diazotrophic cyanobacterium PCC 7942. The Ca transient was triggered in response to nitrogen step-down or the addition of 2-oxoglutarate (2-OG), or its analogues 2,2-difluoropentanedioic acid (DFPA) and 2-methylenepentanedioic acid (2-MPA), to cells growing with combined nitrogen, suggesting that an increase in intracellular 2-OG levels precedes the Ca transient. The signalling protein P and the transcriptional regulator NtcA appear to be needed to trigger the signal. Suppression of the Ca transient by the intracellular Ca chelator ,-[1,2-ethanediylbis(oxy-2,1-phenylene)]bis[-[2-[(acetyloxy)methoxy]-2-oxoethyl]]-,bis[(acetyloxy)methyl] ester (BAPTA-AM) inhibited expression of the and genes, which are involved in acclimation to nitrogen starvation and transcriptionally activated by NtcA. BAPTA-AM treatment partially inhibited expression of the gene, which is involved in phycobiliprotein degradation following nutrient starvation and is regulated by NtcA and NblR; in close agreement, BAPTA-AM treatment partially inhibited bleaching following nitrogen starvation. Taken together, the results presented here strongly suggest an involvement of a defined Ca transient in acclimation of to nitrogen starvation through NtcA-dependent regulation.

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2009-01-01
2021-05-10
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