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Abstract

Acyl carrier proteins (ACPs) are small acidic proteins that carry growing acyl chains during fatty acid or polyketide synthesis. In rhizobia, there are four different and well-characterized ACPs: AcpP, NodF, AcpXL and RkpF. The genome sequence of 1021 reveals two additional ORFs that possibly encode additional ACPs. One of these, , is located on the plasmid pSymB as part of an operon. The genes of the operon encode a putative asparagine synthetase (AsnB), the predicted ACP (SMb20651), a putative long-chain fatty acyl-CoA ligase (SMb20650) and a putative ammonium-dependent NAD synthetase (NadE1). When SMb20651 was overexpressed in [H]-alanine, a biosynthetic building block of 4′-phosphopantetheine, was incorporated into the protein . The purified SMb20651 was modified with 4′-phosphopantetheine in the presence of holo-ACP synthase (AcpS). Also, holo-SMb20651 was modified with a malonyl group by malonyl CoA-ACP transacylase. In , coexpression of SMb20651 together with other proteins such as AcpS and SMb20650 led to the formation of additional forms of SMb20651. In this bacterium, acylation of SMb20651 with C12 : 0 or C18 : 0 fatty acids was detected, demonstrating that this protein is involved in fatty acid biosynthesis or transfer. Expression of SMb20651 was detected in as holo-SMb20651 and acyl-SMb20651.

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2009-01-01
2019-11-15
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