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Volume 155, Issue 4

Regulation of expression during biotin-limited growth of Free

Abstract

is a biotin-auxotrophic bacterium and some strains efficiently produce glutamic acid under biotin-limiting conditions. In an effort to understand metabolism under biotin limitation, growth of the type strain ATCC 13032 was investigated in batch cultures and a time-course analysis was performed. A transient excretion of organic acids was observed and we focused our attention on lactate synthesis. Lactate synthesis was due to the -encoded -lactate dehydrogenase (Ldh). Features of Ldh activity and transcription were analysed. The gene was shown to be regulated at the transcriptional level by SugR, a pleiotropic transcriptional repressor also acting on most phosphotransferase system (PTS) genes. Electrophoretic mobility shift assays (EMSAs) and site-directed mutagenesis allowed the identification of the SugR-binding site. Effector studies using EMSAs and analysis of expression in a mutant revealed fructose 1-phosphate as a highly efficient negative effector of SugR. Fructose 1,6-bisphosphate also affected SugR binding.

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Keyword(s): EMSA, electrophoretic mobility shift assay , Ldh, lactate dehydrogenase , PTS, phosphotransferase system and β-Gal, β-galactosidase
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2009-04-01
2024-04-25
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Regulation of ldh expression during biotin-limited growth of Corynebacterium glutamicum
Microbiology 155, 1360 (2009); https://doi.org/10.1099/mic.0.022004-0
/content/journal/micro/10.1099/mic.0.022004-0
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