@article{mbs:/content/journal/micro/10.1099/mic.0.021931-0, author = "Jandu, Narveen and Zeng, Zoë Jingjing and Johnson-Henry, Kathene C. and Sherman, Philip M.", title = "Probiotics prevent enterohaemorrhagic Escherichia coli O157 : H7-mediated inhibition of interferon-γ-induced tyrosine phosphorylation of STAT-1", journal= "Microbiology", year = "2009", volume = "155", number = "2", pages = "531-540", doi = "https://doi.org/10.1099/mic.0.021931-0", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.021931-0", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "EPEC, enteropathogenic Escherichia coli", keywords = "LEE, locus of enterocyte effacement", keywords = "EHEC, enterohaemorrhagic Escherichia coli", keywords = "STAT-1, signal transducer and activator of transcription-1", keywords = "IFN, interferon", abstract = "Enterohaemorrhagic Escherichia coli (EHEC) O157 : H7 inhibits interferon (IFN)-γ-stimulated tyrosine phosphorylation of signal transducer and activator of transcription (STAT)-1 in epithelial cells. We determined the effects of probiotics on EHEC-mediated disruption of IFN-γ-stimulated STAT-1 activation in epithelial cell lines. Confluent Intestine 407, HEp-2 and Caco-2 epithelial cells were pre-treated (3 h) with either probiotics or surface-layer proteins derived from Lactobacillus helveticus R0052 prior to infection with EHEC O157 : H7 strain CL56 (m.o.i. 100 : 1, 6 h, 37 °C in 5 % CO2). Subsequently, cells were washed and stimulated with human recombinant IFN-γ (50 ng ml−1, 0.5 h, 37 °C) followed by whole-cell protein extraction and immunoblotting for tyrosine-phosphorylated STAT-1. Relative to uninfected cells, STAT-1-activation was reduced after EHEC O157 : H7 infection. Pre-incubation with the probiotic L. helveticus R0052 followed by EHEC infection abrogated pathogen-mediated disruption of IFN-γ–STAT-1 signalling. As determined using Transwell inserts, probiotic-mediated protection was independent of epithelial cell contact. In contrast, pre-incubation with boiled L. helveticus R0052, an equal concentration of viable Lactobacillus rhamnosus R0011, or surface-layer proteins (0.14 mg ml−1) did not restore STAT-1 signalling in EHEC-infected cells. The viable probiotic agent L. helveticus R0052 prevented EHEC O157 : H7-mediated subversion of epithelial cell signal transduction responses.", }