1887

Abstract

The Gram-negative Tol-Pal system of envelope proteins plays a key role in maintaining outer membrane integrity and contributes to the virulence of several pathogens. We have investigated the role of one of these proteins, TolA, in the biology of serovar Typhimurium. Deletion of rendered strain SL1344 more susceptible to killing by bile and human serum. In addition the mutant had impaired membrane integrity and displayed alterations in LPS production. The mutant was highly attenuated in mouse infections via the oral and intravenous routes. Importantly, each phenotype displayed by the mutant was complemented by provision of . The gene therefore contributes to virulence, membrane integrity, LPS production and bile and serum resistance in serovar Typhimurium SL1344. Finally, immunization with the mutant provided significant protection against subsequent challenge with wild-type SL1344. The Tol-Pal system is therefore a potential target in the development of novel attenuated live vaccines against and other Gram-negative pathogens.

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2009-01-01
2020-01-20
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