1887

Abstract

The bioprotection performance of against the root parasite was studied. We found that maize plants first grown with and at day 10 inoculated with showed improvements in biomass, and root length and number as compared with plants grown with alone. To validate our finding that inoculation with suppresses colonization by , we performed PCR analyses using - and -specific primers. Our results showed that inoculation with suppresses further colonization by . We hypothesized that as the colonization by increases, the presence of/colonization by decreases. In roots, catalase (CAT), glutathione reductase (GR), glutathione -transferase (GST) and superoxide dismutase (SOD) activities were found to be higher in -colonized plants than in non-colonized plants. Increased activity of antioxidant enzymes minimizes the chances of oxidative burst (excessive production of reactive oxygen species), and therefore might be protected from the oxidative defence system during colonization. We also observed decreased antioxidant enzyme activities in plants first inoculated with and at day 10 inoculated with as compared with plants inoculated with alone. These decreased antioxidant enzyme activities due to the presence of help the plant to overcome the disease load of . We propose that can be used as a bioprotection agent against the root parasite

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2009-03-01
2020-07-09
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References

  1. Alguacil M. M., Hernandez J. A., Caravaca F., Portillo B., Roldan A.. 2003; Antioxidant enzyme activities in shoots from three mycorrhizal shrub species afforested in a degraded semi-arid soil. Physiol Plant118:562–570
    [Google Scholar]
  2. Alvarez M. E., Pennell R. I., Meijer P. J., Ishikawa A., Dixon R. A., Lamb C.. 1998; Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity. Cell92:773–784
    [Google Scholar]
  3. Arnon D. I., Hoagland D. R.. 1940; Crop production in artificial solutions and soil with special reference to factors influencing yields and absorption of organic nutrients. Soil Sci50:463–484
    [Google Scholar]
  4. Auh C. K., Murphy T. M.. 1995; Plasma membrane redox enzyme is involved in the synthesis of O2 and H2O2 by Phytophthora elicitor-stimulated rose cells. Plant Physiol107:1241–1247
    [Google Scholar]
  5. Beers R. F., Sizer I. W.. 1952; A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem195:133–140
    [Google Scholar]
  6. Blilou I., Bueno P., Ocampo J. A., Garcia-Garrido J.. 2000; Induction of catalase and ascorbate peroxidase activities in tobacco roots inoculated with the arbuscular mycorrhizal Glomus mosseae. Mycol Res104:722–725
    [Google Scholar]
  7. Bolwell G. P., Butt V. S., Davies D. R., Zimmerlin A.. 1995; The origin of the oxidative burst in plants. Free Radic Res23:517–532
    [Google Scholar]
  8. Boukcim H., Plassard C.. 2003; Juvenile nitrogen uptake capacities and root architecture of two open-pollinated families of Picea abies. Effects of nitrogen source and ectomycorrhizal symbiosis. J Plant Physiol160:1211–1218
    [Google Scholar]
  9. Bousquet J., Simon L., LaLonde M.. 1990; DNA amplification from vegetative and sexual tissues of trees using polymerase chain reaction. Can J Res20:254–257
    [Google Scholar]
  10. Bradford M. M.. 1976; A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem72:248–254
    [Google Scholar]
  11. Christensen J. J., Wilcoxson R. D.. 1966; Stalk Rot in Corn. The American Phytopathological Society, monograph no. 3 St Paul, MN: The American Phytopathological Society;
    [Google Scholar]
  12. Corpas F. J., Barroso J. B., del Rio L. A.. 2001; Peroxisomes as a source of reactive oxygen species and nitric oxide signal molecules in plant cells. Trends Plant Sci6:145–150
    [Google Scholar]
  13. Croft K. P. C., Voisey C. R., Slusarenko A. J.. 1990; Mechanism of hypersensitive cell collapse: correlation of increased lipoxygenase activity with membrane damage in leaves of Phaseolus vulgaris (L.) inoculated with an avirulent race of Pseudomonas syringae pv. phaseolicola. Physiol Mol Plant Pathol36:49–62
    [Google Scholar]
  14. Daniell T. J., Husband R., Fitter A. H., Young J. P. W.. 2001; Molecular diversity of arbuscular mycorrhizal fungi colonising arable crops. FEMS Microbiol Ecol36:203–209
    [Google Scholar]
  15. Daniels B. A.. 1983; Elimination of Fusarium moniliforme from corn seed. Plant Dis67:609–611
    [Google Scholar]
  16. Dean J. D., Goodwin P. H., Hsiang T.. 2005; Induction of glutathione S-transferase genes of Nicotiana benthamiana following infection by Colletotrichum destructivum and C. orbiculare and involvement of one in resistance. J Exp Bot56:1525–1533
    [Google Scholar]
  17. De Gara L., De Pinto M. C., Tommasi F.. 2003; The antioxidant systems vis-à-vis reactive oxygen species during plant–pathogen interaction. Plant Physiol Biochem41:863–870
    [Google Scholar]
  18. Deshmukh S., Huckelhoven R., Schafer P., Imani J., Sharma M., Weiss M., Waller F., Kogel K. H.. 2006; The root endophytic fungus Piriformospora indica requires host cell death for proliferation during mutualistic symbiosis with barley. Proc Natl Acad Sci U S A103:18450–18457
    [Google Scholar]
  19. Dickson S., Mandeep Smith S. M.. 1998; Evaluation of vesicular arbuscular mycorrhizal colonization by staining. In Mycorrhiza Manual pp77–84 Edited by Varma A.. Berlin/Heidelberg: Springer Verlag;
    [Google Scholar]
  20. Doke N.. 1983; Involvement of superoxide anion generation in the hypersensitive response of potato tuber tissues to infection with an incompatible race of Phytophthora infestans and to the hyphal wall components. Physiol Plant Pathol23:345–357
    [Google Scholar]
  21. Doke N., Miura Y.. 1995; In vitro activation of NADPH-dependent O2 generating system in a plasma membrane-rich fraction of potato tuber tissues by treatment with an elicitor from Phytophthora infestans or with digitonin. Physiol Mol Plant Pathol46:17–28
    [Google Scholar]
  22. Futrell M. C., Kilgore M.. 1969; Poor stands of corn and reduction of root growth caused by Fusarium verticillioides. Plant Dis Rep53:213–215
    [Google Scholar]
  23. García-Garrido J. M., Ocampo J. A.. 2002; Regulation of the plant defense response in arbuscular mycorrhizal symbiosis. J Exp Bot53:1377–1386
    [Google Scholar]
  24. Gianinazzi-Pearson V.. 1996; Plant cell responses to arbuscular mycorrhizal fungi: getting to the roots of the symbiosis. Plant Cell8:1871–1883
    [Google Scholar]
  25. Gosling P., Hodge A., Goodlass G., Bending G. D.. 2006; Arbuscular mycorrhizal fungi and organic farming. Agric Ecosyst Environ113:17–35
    [Google Scholar]
  26. Goyal V., Chetal S., Nainawatee H. S.. 1986; Alterations in Rhizobium trifolii catalase under water stress. Folia Microbiol31:164–166
    [Google Scholar]
  27. Grant J. J., Loake G. J.. 2000; Role of reactive oxygen intermediates and cognate redox signalling in disease resistance. Plant Physiol124:21–29
    [Google Scholar]
  28. Grunwald U., Nyamsuren O., Tarnasloukht M., Lapopin L., Becker A., Mann P., Gianinazzi-Pearson V., Krajinski F., Franken P.. 2004; Identification of mycorrhiza-regulated genes with arbuscule development-related expression profile. Plant Mol Biol55:553–566
    [Google Scholar]
  29. Guescini M., Pierleoni R., Palma F., Zeppa S., Vallorani L., Potenza L., Sacconi C., Giomaro G., Stocchi V.. 2003; Characterization of the Tuber borchii nitrate reductase gene and its role in ectomycorrhizae. Mol Genet Genomics269:807–816
    [Google Scholar]
  30. Habig W. H., Pabst M. J., Jacoby W. B.. 1974; Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem249:7130–7139
    [Google Scholar]
  31. Harrier L. A., Watson C. A.. 2004; The potential role of arbuscular mycorrhizal (AM) fungi in the bioprotection of plants against soil-borne pathogens in organic and/or other sustainable farming systems. Pest Manag Sci60:149–157
    [Google Scholar]
  32. Harrison M. J.. 1997; The arbuscular mycorrhizal symbiosis: an underground association. Trends Plant Sci2:54–60
    [Google Scholar]
  33. Hill T. W., Kaefer E.. 2001; Improved protocols for aspergillus medium: trace elements and minimum medium salt stock solutions. Fungal Genet News Lett48:20–21
    [Google Scholar]
  34. Ingram D. S.. 1978; Cell death and resistance to biotrophs. Ann Appl Biol89:291–295
    [Google Scholar]
  35. Kogel K. H., Franken P., Huckelhovenl R.. 2006; Endophyte or parasite – what decides?. Curr Opin Plant Biol9:358–363
    [Google Scholar]
  36. Lambais M. R., Mehdy M. C.. 1993; Suppression of endochitinase, β-1,3-endoglucanase, and chalcone isomerase expression in bean vesicular-arbuscular mycorrhizal roots under different soil phosphate conditions. Mol Plant Microbe Interact6:75–83
    [Google Scholar]
  37. Lambais M. R., Mehdy M. C.. 1995; Differential expression of defense-related genes in arbuscular mycorrhiza. Can J Bot73:S533–S540
    [Google Scholar]
  38. Lambais M. R., Mehdy M. C.. 1998; Spatial distribution of chitinases and β-1,3-glucanase transcripts in bean arbuscular mycorrhizal roots under low and high soil phosphate conditions. New Phytol140:33–42
    [Google Scholar]
  39. Levine A., Tenhaken R., Dixon R., Lamb C.. 1994; H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response. Cell79:583–593
    [Google Scholar]
  40. McGonigle T. P., Miller M. H., Evans D. G., Fairchild G. L., Swan J. A.. 1990; A new method which gives an objective measure of colonization of roots by vesicular-arbuscular mycorrhizal fungi. New Phytol115:495–501
    [Google Scholar]
  41. Mehdy M., Sharma Y. K., Sathasivan K., Bays N. W.. 1996; The role of activated oxygen species in plant disease resistance. Physiol Plant98:365–374
    [Google Scholar]
  42. Mittler R.. 2002; Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci7:405–410
    [Google Scholar]
  43. Ni M., Dehesh K., Tepperman J. M., Quail P. H.. 1996; GT-2: In vivo transcriptional activation activity and definition of novel twin DNA binding domains with reciprocal target sequence selectivity. Plant Cell8:1041–1059
    [Google Scholar]
  44. Nordhoff A., Bucheler U. S., Werner D., Schirmer R. H.. 1993; Folding of the four domains and dimerization are impaired by the Gly446→Glu exchange in human glutathione reductase. Implications for the design of antiparasitic drugs. Biochemistry32:4060–4066
    [Google Scholar]
  45. Oehl F., Sieverding E., Mader P., Dubois D., Ineichen K., Boller T., Wiemken A.. 2004; Impact of long-term conventional and organic farming on the diversity of arbuscular mycorrhizal fungi. Oecologia138:574–583
    [Google Scholar]
  46. Peskan-Berghofer T., Shahollari B., Giong P. H., Hehl S., Markert C., Blanke V., Kost G., Varma A., Oelmuller R.. 2004; Association of Piriformospora indica with Arabidopsis thaliana roots represents a novel system to study beneficial plant–microbe interactions and involves early plant protein modifications in the endoplasmic reticulum and at the plasma membrane. Physiol Plant122:465–477
    [Google Scholar]
  47. Phillips J. M., Hayman D. S.. 1970; Improved procedures for clearing roots and staining parasitic and VAM fungi for rapid assessment of infection. Trans Br Mycol Soc55:158–161
    [Google Scholar]
  48. Plenchette C., Clermont-Dauphin C., Meynard J. M., Fortin J. A.. 2005; Managing arbuscular mycorrhizal fungi in cropping systems. Can J Plant Sci85:31–40
    [Google Scholar]
  49. Porcel R., Barea J. M., Ruiz-Lozano J. M.. 2003; Antioxidant activities in mycorrhizal soybean plants under drought stress and their possible relationship to the process of nodule senescence. New Phytol157:135–143
    [Google Scholar]
  50. Requena N., Perez-Solis E., Azcon-Aguilar C., Jeffries P., Barea J. M.. 2001; Management of indigenous plant–microbe symbioses aids restoration of desertified ecosystems. Appl Environ Microbiol67:495–498
    [Google Scholar]
  51. Roth E. F. Jr, Gilbert H. S.. 1984; Pyrogallol assay for SOD: absence of a glutathione artifact. Anal Biochem137:50–53
    [Google Scholar]
  52. Ruiz-Lozano J. M., Azcón R., Palma J. M.. 1996; Superoxide dismutase activity in arbuscular mycorrhizal Lactuca sativa plants subjected to drought stress. New Phytol134:327–333
    [Google Scholar]
  53. Scott G. S., Futrell M. C.. 1970; Response of maize seedlings to Fusarium moniliforme and a toxic material extracted from this fungus. Pl Dis Reporter54:483–486
    [Google Scholar]
  54. Serfling A., Wirsel S. G. R., Lind V., Deising H. B.. 2007; Performance of the biocontrol fungus Piriformospora indica on wheat under greenhouse and field conditions. Phytopathology97:523–531
    [Google Scholar]
  55. Sherameti I., Shahollari B., Venus Y., Altschmied L., Varma A., Oelmuller R.. 2005; The endophytic fungus Piriformospora indica stimulates the expression of nitrate reductase and the starch-degrading enzyme glucan-water dikinase in tobacco and Arabidopsis roots through a homeodomain transcription factor that binds to a conserved motif in their promoters. J Biol Chem280:26241–26247
    [Google Scholar]
  56. Tanaka A., Christensen M. J., Takemoto D., Park P., Scotta B.. 2006; Reactive oxygen species play a role in regulating a fungus–perennial ryegrass mutualistic interaction. Plant Cell18:1052–1066
    [Google Scholar]
  57. Tenhaken R., Levine A., Brisson L. F., Dixon R., Lamb C.. 1995; Function of the oxidative burst in hypersensitive disease resistance. Proc Natl Acad Sci U S A92:4158–4163
    [Google Scholar]
  58. Toro M., Azcon R., Barea J. M.. 1998; The use of isotopic dilution techniques to evaluate the interactive effects of Rhizobium genotype, mycorrhizal fungi, phosphate-solubilizing rhizobacterias and rock phosphate on nitrogen and phosphorus acquisition by Medicago sativa. New Phytol138:265–273
    [Google Scholar]
  59. Vanacker H., Harbinson J., Ruisch J., Carver T. L. W., Foyer C. H.. 1998; Antioxidant defences of the apoplast. Protoplasma205:129–140
    [Google Scholar]
  60. Varma A., Verma S., Sudha, Sahay N., Butehorn B., Franken P.. 1999; Piriformospora indica, a cultivable plant-growth-promoting root endophyte. Appl Environ Microbiol65:2741–2744
    [Google Scholar]
  61. Varma A., Singh A., Sudha, Sahay N. S., Sharma J., Roy A., Kumari M., Rana D., Thakran S.. other authors 2001; Piriformospora indica: an axenically culturable mycorrhiza-like endosymbiotic fungus. In The Mycota IX, Fungal Associations pp125–150 Edited by Hock B. Berlin-Heidelberg: Springer Verlag;
    [Google Scholar]
  62. Verma S., Varma A., Rexer K.-H., Hassel A., Kost G., Sarbhoy A., Bisen P., Buetehorn B., Franken P.. 1998; Piriformospora indica, gen. et sp. nov., a new root colonizing fungus. Mycologia90:896–903
    [Google Scholar]
  63. Waller F., Baltruschat H., Achatz B., Becker K., Fischer M., Fodor J., Heier T., Huckelhoven R., Neumann C.. other authors 2005; The endophytic fungus Piriformospora indica reprograms barley to salt-stress tolerance, disease resistance, and higher yield. Proc Natl Acad Sci U S A102:13386–13391
    [Google Scholar]
  64. Wojtaszek P.. 1997; Oxidative burst: an early plant response to pathogen infection. Biochem J322:681–692
    [Google Scholar]
  65. Wu G. S., Shortt B. J., Lawrence E. B., Leon J., Fitzsimmons K. C., Levine E. B., Raskin I., Shah D. M.. 1997; Activation of host defense mechanisms by elevated production of H2O2 in transgenic plants. Plant Physiol115:427–435
    [Google Scholar]
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