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Volume 172, Issue 2

D-amino acid substitution and cyclization enhance the stability and antimicrobial activity of arginine-rich peptides Open Access

Abstract

Cationic peptides, particularly those rich in arginine and/or lysine residues, are usually promising antimicrobial agents effective at low concentrations in laboratory settings. However, their applicability in pharmaceutics and biotechnology is currently limited due to their susceptibility to biological enzymatic processes and (in some cases) toxicity to host cells. To address this, we screened eight linear arginine-rich peptides for their haemolytic properties and antimicrobial activity using a set of computational and experimental assays. Inspired by our previous results on R4F4, we then designed three modified peptides based on an R4F4 backbone, R4F4-C16, -R4F4 and cyclic R4F4, and one based on R4 (R4-C16). Amongst the tested linear peptides containing only natural amino acids, R4F4 exhibited the strongest antibacterial activity; however, this effect was reduced in the presence of human serum and trypsin. Conversely, our study demonstrated that cyclization and substitution to its -amino acid enantiomer significantly enhanced stability and activity of R4F4, whilst in the presence of proteases. As revealed by fluorescence imaging, microscopy RNA sequencing analysis, the mode of action involves complex and dynamic events. This multifaceted antimicrobial mechanism integrates alterations in membrane permeability, modulation of intracellular reactive oxygen species levels and changes in transcriptomic signature profiles. At the molecular level, notable changes were observed in the bacterial expression of genes associated with metabolic pathways and biological processes. Furthermore, R4F4-derived peptides showed substantial antibiofilm activity in preventing the formation and disruption of mature biofilms, together with good cytocompatibility, highlighting the potential for clinical applicability. In conclusion, this study emphasizes the importance of optimizing the stability of peptide-based antimicrobials, particularly those rich in arginine, and highlights the advantages of incorporating -amino acids and cyclization for enhanced performance. This information will prove useful in the future design of antimicrobial peptides. In addition, the molecular perspective on peptide-induced gene expression changes, as identified by RNA-seq, broadens our understanding of antimicrobial peptides’ activities and provides a clearer picture of their versatile mechanisms.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): antibacterial activity , arginine-rich peptide , cationic peptide and R4F4 antimicrobial peptide
Funding
This study was supported by the:
  • UK Research and Innovation (Award EP/V053396/1)
    • Principal Award Recipient: IanW. Hamley
© 2026 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2026-02-04
2026-02-07

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/content/journal/micro/10.1099/mic.0.001657
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D-amino acid substitution and cyclization enhance the stability and antimicrobial activity of arginine-rich peptides
Microbiology 172, 001657 (2026); https://doi.org/10.1099/mic.0.001657
/content/journal/micro/10.1099/mic.0.001657
/content/journal/micro/10.1099/mic.0.001657
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