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Volume 170, Issue 11

Fluoride and gallein regulate polyphosphate accumulation in dental caries-associated Open Access

Abstract

Inorganic polyphosphates (polyPs) are energy-storing biopolymers synthesized by all three domains of life. PolyP accumulation has been well studied with respect to its role in stress response, but its role in dental disease has received less attention. Dental decay can be promoted by changes in pH as well as the chemical activity of ions such as phosphate in oral fluids at the enamel interface. Previous work has shown that the drawdown of phosphate from biofilm fluids can alter the saturation state of oral fluids to thermodynamically favour mineral dissolution. The members of the Lactobacillaceae are known to accumulate polyP and play a role in early-stage and late-stage dental caries. In this study, we examined the effects of potential metabolic inhibitors on polyP accumulation in . We observed that two inhibitors of the enzyme responsible for polyP synthesis, gallein and fluoride, inhibited polyP accumulation in a balanced medium. However, fluoride and gallein treatments amended with either glucose or lactate were found to enhance polyP accumulation. These results illustrate the potential complexity of polyP metabolism in the oral environment.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): caries , fluoride , gallein , PEP-PTS and polyphosphate
Funding
This study was supported by the:
  • National Institute of Dental and Craniofacial Research (Award 1R01DE027669-01)
    • Principal Award Recipient: JakeBailey
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2025-12-12

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/content/journal/micro/10.1099/mic.0.001519
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Fluoride and gallein regulate polyphosphate accumulation in dental caries-associated Lacticaseibacillus
Microbiology 170, 001519 (2024); https://doi.org/10.1099/mic.0.001519
/content/journal/micro/10.1099/mic.0.001519
/content/journal/micro/10.1099/mic.0.001519
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