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Volume 170, Issue 10

Biotin concentration affects anaplerotic reactions functioning in glutamic acid production in Open Access

Abstract

The study investigates the effect of biotin concentration on the role of anaplerotic reactions catalysed by pyruvate carboxylase (PC) and phosphoenolpyruvate carboxylase (PEPC) in glutamic acid production by requires biotin for its growth, and its glutamic acid production can be induced by the addition of Tween 40 or penicillin or by biotin limitation. The biotin enzyme PC and the non-biotin enzyme PEPC catalyse two anaplerotic reactions to supply oxaloacetic acid to the TCA cycle in . Therefore, they are crucial for glutamic acid production in this bacterium. In this study, we investigated the contribution of each anaplerotic reaction to Tween 40- and penicillin-induced glutamic acid production using disruptants of PEPC and PC. In the presence of 20 µg l biotin, which is sufficient for growth, the PEPC-catalysed anaplerotic reaction mainly contributed to Tween 40- and penicillin-induced glutamic acid production. However, when increasing biotin concentration 10-fold (i.e. 200 µg l), both PC- and PEPC-catalysed reactions could function in glutamic acid production. Western blotting revealed that the amount of biotin-bound PC was reduced by the addition of Tween 40 and penicillin in the presence of 20 µg l. However, these induction treatments did not change the amount of biotin-bound PC in the presence of 200 µg l biotin. These results indicate that both anaplerotic reactions are functional during glutamic acid production in and that biotin concentration mainly affects which anaplerotic reactions function during glutamic acid production.

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Keyword(s): anaplerotic reaction , biotin , Corynebacterium glutamicum , glutamic acid , phosphoenolpyruvate carboxylase and pyruvate carboxylase
Funding
This study was supported by the:
  • Japan Society for the Promotion of Science (Award JP16K14881)
    • Principal Award Recipient: TakashiHirasawa
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-10-07
2025-11-18

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/content/journal/micro/10.1099/mic.0.001507
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Biotin concentration affects anaplerotic reactions functioning in glutamic acid production in Corynebacterium glutamicum
Microbiology 170, 001507 (2024); https://doi.org/10.1099/mic.0.001507
/content/journal/micro/10.1099/mic.0.001507
/content/journal/micro/10.1099/mic.0.001507
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