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Volume 170, Issue 9

A comparative genomic and phenotypic study of model strains using hybrid sequencing Open Access

Abstract

Next-generation sequencing methods have become essential for studying bacterial biology and pathogenesis, often depending on high-quality, closed genomes. In this study, we utilized a hybrid sequencing approach to assemble the genome of C6706, a widely used model strain. We present a manually curated annotation of the genome, enhancing user accessibility by linking each coding sequence to its counterpart in N16961, the first sequenced isolate and a commonly used reference genome. Comparative genomic analysis between C6706 and N16961 uncovered multiple genetic differences in genes associated with key biological functions. To determine whether these genetic variations result in phenotypic differences, we compared several phenotypes relevant to pathogenicity like genetic stability, acid sensitivity, biofilm formation and motility. Notably, N16961 exhibited greater motility and reduced biofilm formation compared to C6706. These phenotypic differences appear to be mediated by variations in quorum sensing and cyclic di-GMP signalling pathways between the strains. This study provides valuable insights into the regulation of biofilm formation and motility in .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): biofilm , c-di-GMP signalling , hybrid sequencing , motility , QS and V. cholerae
Funding
This study was supported by the:
  • Norges Forskningsråd (Award 249979)
    • Principle Award Recipient: SörenAbel
© 2024 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2024-09-23
2024-10-14
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/content/journal/micro/10.1099/mic.0.001502
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A comparative genomic and phenotypic study of Vibrio cholerae model strains using hybrid sequencing
Microbiology 170, 001502 (2024); https://doi.org/10.1099/mic.0.001502
/content/journal/micro/10.1099/mic.0.001502
/content/journal/micro/10.1099/mic.0.001502
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